FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1789_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1789_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339660
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT23240.17347685233566726No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC16470.12294164190914111No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC13450.10039860860218265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC1301.2914825E-1050.2908821
CTACGCA1651.3515091E-939.5905382
CCGACGC850.002745577834.109375145
GAGCGAA2455.4569682E-1132.614951
AGCGAAA5400.032.2855031
GATAGCG2451.9790605E-929.584661145
GCGAAAG6000.029.0330622
CGAAAGC6400.027.1961333
TATACTG7250.026.9974825
ACTCCGA3005.7480065E-1026.6355421
CCGAGGT3357.4578566E-1125.9696674
ACATGGT6700.025.967735
AGCACCG15800.024.7761735
AGACGAC2051.2875182E-524.7639583
CGACTCC2051.2919969E-524.7528656
CGCACTG2951.4302714E-824.573985
GCACCGT16300.024.4599956
ACGACTC2202.2311433E-523.066035
CACCGTG18200.022.7013857
ACCGTGT17500.022.3660038
Produced by FastQC (version 0.11.5)