FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1789_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1789_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142410
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT50390.23520241223668673No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC50050.2336154144164749No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC31010.1447435364846131No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT29510.1377420755130904No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC25740.12014507027132997No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA25700.11995836464542267No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC24970.11655098697261496No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC24940.1164109577531845No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA24500.11435719586820449No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA22750.10618882473476132No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22310.10413506284978132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC2700.053.804161
CTACGCA2950.049.2007642
GCCGACC1551.4551915E-1146.766537145
CGATGTT803.738675E-545.3071945
GTCGGCT1807.2759576E-1140.2711836
AAGGTCG5950.037.768695
AGCGAAA7900.037.6969681
GGTCGTT6000.037.451337
GACGACT1553.473542E-837.4193464
CGACTCC1752.4356268E-937.2796136
TAACCGT1251.2667759E-534.795935
GCGAAAG9050.034.4813042
GTCGTTT7150.033.455288
CCGACTA3350.032.4626774
CGAAAGC9400.032.405713
TATACTG19250.032.3858225
TACGCAC4500.032.234253
ACGACTC1851.6468039E-731.3476815
CGCACTG5200.030.6694875
AGGTCGT7400.030.3666486
Produced by FastQC (version 0.11.5)