FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1788_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1788_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926564
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG8250.039.5385329
TATCGTC1209.581643E-636.2436527
TATATCG2054.0455961E-728.2911155
CGAAAGC22500.026.755763
CGTACGG1658.427194E-526.3621735
AGCGAAA23300.025.5642761
ACCGTTA2051.29312375E-524.7517648
CATAGTG14600.024.3325044
CTGCCGC14700.024.162436145
GCGAAAG25650.024.0540122
GTATTAT21000.023.521511
AGTGATC17300.023.0450977
GTCATAG16400.022.5726382
CGTACGC2252.657391E-522.551607145
ACTCCGC6050.021.566478
TACGCAG2704.4518383E-621.480295
ATAGTGA17750.021.2382585
GTATATA19850.020.4928841
TATATAC17900.020.2599663
GCTATAT10050.020.2379971
Produced by FastQC (version 0.11.5)