FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1788_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1788_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757628
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAAT105400.5996718304442124No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT97540.5549524700334769No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG92950.5288377290302613No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC81280.4624414267410396No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA71490.4067413582396275No Hit
GTCTTCCGACTGGGCTCTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT70690.4021897693937512No Hit
GTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGGCGTTG66170.37647329241454963No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG63890.36350126420380197No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA59590.33903647415721644No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA51620.29369127028017306No Hit
GTCCTGTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTC49770.28316572107408394No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGA48210.274290122824625No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG47530.27042127230563007No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT45550.25915608991208605No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGC43860.2495408584751722No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA41850.23810499149990783No Hit
CCCATATACAGCGCATCTGGATGCACCAGACGGCAGTCGCGCATGAAGAT40700.23156208253396052No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT40000.22757944229381874No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG39740.2261001759189089No Hit
GGTCAGAACAGGACGTGTTGATAAAACAGAATTTGCCTGGCGGCCGTAGC38770.2205813744432838No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG38350.2181917902991987No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT37290.2121609350784125No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG37280.21210404021783902No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA35500.2019767550357641No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACA34980.19901822228594446No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG34230.19475110774293536No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT34020.19355631567089282No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC33530.19076846750279355No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT32710.18610308893577027No Hit
CTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG32580.18536345574831534No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG32450.18462382256086043No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC30940.17603269861426876No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG29760.1693191050666011No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG29600.16840878729742587No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA29550.16812431299455857No Hit
GTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG29440.16749846952825057No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA29260.1664743620379284No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG29010.16505199052359204No Hit
GGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT28770.1636865138698291No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT28630.16288998582180073No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC28510.16220724749491927No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG28010.15936250446624656No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA27050.1539005978511949No Hit
CTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG26600.15134032912538944No Hit
GGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAACAC26030.14809732207270251No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG25780.14667495055836616No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT25780.14667495055836616No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA25660.1459922122314847No Hit
GATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATT25530.1452525790440298No Hit
CCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGG25250.14365952294797307No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG25210.14343194350567925No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA24800.1410992542221676No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA24580.13984756728955158No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAC24530.13956309298668432No Hit
GCCTGATACAGATTAAATCGACAGGTCAGAACAGGACGTGTTGATAAAAC24520.13950619812611087No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT24520.13950619812611087No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT24510.13944930326553742No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA24430.13899414438094979No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC24120.1372304037031727No Hit
GCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCTCT23810.1354666630253956No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT23700.13484081955908758No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG23110.13148402278525376No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG22880.1301754409920643No Hit
CTTCAGGACCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCG22750.1294358078046094No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC22650.12886685919887486No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC22520.12812722601141996No Hit
ATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAC22520.12812722601141996No Hit
AAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGC22380.12733069796339158No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG22010.12522558812217377No Hit
GTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGC21750.12374632174726394No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC21540.12255152967522139No Hit
GCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATC21330.12135673760317882No Hit
GAACAGGACGTGTTGATAAAACAGAATTTGCCTGGCGGCCGTAGCGCGGT21270.1210153684397381No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT20810.1183982048533592No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC20780.11822752027163881No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT20510.11669135903615556No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT20110.11441556461321736No Hit
CTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTG19980.11367593142576245No Hit
GGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCAT19970.11361903656518899No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT19860.11299319309888099No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA19650.11179840102683845No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG19520.11105876783938354No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC19470.11077429353651626No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCAC19120.10878297341644534No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTCAAGGAGTC18830.10713302245981517No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGTGCGGCTGCGGCGTGG18710.10645028413293371No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA18680.10627959955121333No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT18640.10605202010891951No Hit
GATTAAATCGACAGGTCAGAACAGGACGTGTTGATAAAACAGAATTTGCC18460.10502791261859734No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT18300.10411759484942205No Hit
CATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAACAACATA18140.10320727708024678No Hit
GTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGA17900.10184180042648389No Hit
GTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCT17870.1016711158447635No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT17820.10138664154189625No Hit
CCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGA17700.10070390321501477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAGT17650.078.894863
CATAGTG17650.078.87694
GTCATAG18450.075.521242
CGAAAGC13600.069.859353
GGTCATA21500.065.538841
TGATCCG22850.059.9560479
ATAGTGA23550.058.801225
CGGTAGA3400.051.2705271
TAGTGAT27000.051.01346
GAGCGAA2850.048.422171
TACGAGC1551.4551915E-1146.766846145
AGTGATC30400.045.304097
GTGATCC31550.043.6527528
GGACGGA6950.042.7654956
GTTAAGG9600.041.5772442
AGGACGG7900.038.5448275
TAAGGAT7950.038.3067674
AGCGAAA26100.037.5689241
GACGGAT8600.034.5575377
GACTGAC2353.45608E-1133.929877
Produced by FastQC (version 0.11.5)