FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1788_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1788_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559903
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1051.6370905E-1162.130028145
AGCGAAA6750.045.1988641
GCGAAAG7600.040.112812
CGAAAGC8300.036.705063
TATACTG10400.031.3687425
CGACGAA1050.007717524727.613344145
GTAGCAC5700.025.4512923
GTATGAG7400.022.5776181
GCATACG2303.0966665E-522.108141
GGGTTGA13400.020.017096
GTGTAGG13750.019.547041
CAGTTCG6251.8189894E-1218.5561689
CGCGGCA400.00793021318.121258140-144
TAGCACT11250.018.0459254
TCTATAC16900.018.0267433
GTCCTAT11850.017.777131
ATACTGG10200.017.7682176
GAGCGAA4104.528065E-717.7173231
AGACTGC10650.017.6981456
TAGACAG14550.016.9407985
Produced by FastQC (version 0.11.5)