FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1788_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1788_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324461
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5250.051.12982
AGCGAAA5350.048.8605041
CGAAAGC6150.044.798273
GGTACTG7000.032.1070255
ACCGTGC950.004730548230.5227498
GGATAGC5150.029.5635
AGCACCG3503.6379788E-1226.9284745
CGTTTAT1958.652847E-626.0658971
TCTATAC12250.025.4498483
CAGGTAC10050.023.8067823
GTACTGA13050.023.3314866
TGGATAG6550.023.244194
ACTGATC10400.023.002128
GCAGGTA9400.022.382152
AACCGTG1952.6167659E-422.3059256
CTATACC11150.022.1075444
AGGTACT10900.021.9494614
GTATGGA9500.021.4014721
GTATAAG5301.8189894E-1220.5506061
TATACTG6750.020.4073335
Produced by FastQC (version 0.11.5)