FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1788_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1788_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228071
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4250.044.3979642
AGCGAAA4600.039.477571
ACATCGT1001.3923334E-436.2469026
CGAAAGC5900.031.9594363
GAGCGAA2558.731149E-1131.3343561
TCGCCAT2351.2641976E-930.847174145
TACATCG1358.0858765E-426.8506535
GCCGACC2207.4965465E-726.360313145
CGGTTAG1851.8074227E-423.558511
CATCGTC2252.6547634E-522.5527137
CTGCCGC3901.2219971E-820.44614145
CCGTACA1800.00428239520.1593322
GTATAAT7600.020.071231
TCAGGCG2908.261E-619.9974789
ATGCGTC400.00791370818.12773365-69
GTGCGTA2000.00792283618.1227159
GTGTAAG10100.017.9798851
GATATAT9500.017.5862181
GTATTAT15750.017.5255361
GTGTAGG6705.456968E-1217.3465631
Produced by FastQC (version 0.11.5)