FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1788_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1788_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475383
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18300.12403558940288725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1650.065.8969359
CGAAAGC6850.045.5226143
CCGACGC2200.042.83446145
GCGAAAG7400.041.1871222
TATATCG951.0292194E-438.1573225
AGCGAAA8400.036.3134651
GCCGACC2600.036.244545145
CTACGAC1051.8513524E-434.5326583
TACGACA1100.00967055426.365034
TACTCCG1100.0096737526.3632415
TCGCCAT4500.025.773897145
TGTACCG3151.035005E-925.317085
ACCTGCG6450.023.6002987
TACGTTG2802.3445318E-723.3032235
TACACCG2552.7088845E-622.7447595
TCTATAC18000.022.1584553
CTGCCGC5051.8189894E-1221.531412145
CCTATAC2704.437359E-621.4869883
GTATACA10850.020.7505511
CCCGATA2505.983891E-520.296944145
Produced by FastQC (version 0.11.5)