FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1787_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1787_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139181
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG11050.032.8388252
TATACGG2455.638867E-1132.5493855
AGCGAAA11550.031.4448151
CTGCCGC11700.030.358818145
CGAAAGC13550.027.2934063
CGAATAC2559.513133E-825.584503145
GTGATCG4353.6379788E-1223.3295579
GTATTAT36450.020.9243891
CCGACTA3901.2219971E-820.4486664
GTATATA18750.019.7574041
GCACCGT15300.018.4782856
TACGGAC3252.2081791E-517.843557
TTATACG2901.8670113E-417.4999564
ACCGTGT17900.017.4133838
GTATTAG14900.016.5750051
AGCACCG17250.016.390485
CACCGTG20900.016.3014497
GTATACA18200.015.9642911
GTATAAT14150.015.9135211
TATAATA28050.015.523812
Produced by FastQC (version 0.11.5)