FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1787_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1787_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108537
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC2400.042.286915145
TAATCGT1001.3930872E-436.2450689
AGCGAAA9000.034.7016531
GCGAAAG9050.034.475532
CGAAAGC10100.031.588853
GATAGCG3100.030.39981145
GTCTACG1000.0060224629.0525471
GTTGACA11750.028.4344061
GTGATCG2054.0475425E-728.2888349
GCGATTA1804.7073245E-628.191275145
TATACTG11950.027.3007775
TATGGAC7000.026.92815
GTTACGT3056.9667294E-1026.194921
CGGAGTA2901.19216566E-825.0001544
TATAGAC9550.024.3129522
GCTACTA3301.7789716E-924.163389
CTGCCGC6600.023.06559145
GTATTAT29400.022.975231
ACATGGT14900.021.895595
TAGCATG21050.021.7159842
Produced by FastQC (version 0.11.5)