FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1787_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1787_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176232
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA400.006054596554.367172145
ATAACCG907.485846E-540.2754065
GGTGCGA750.001484542638.66118
GCGTTAA951.0298737E-438.1524059
GCCGACC951.0298737E-438.152405145
TACTGCG1102.4372526E-432.9526025
AACCGTA1102.4384694E-432.94987
TGCGTTA1153.1651068E-431.51728
CTGCCGC3300.030.753149145
TACCGTC1204.0588665E-430.2078384
TAGCATG9700.029.9269522
AGTCCGC1703.0156916E-629.848644145
GTTGACA7000.029.0526371
GAATCGC1504.3452797E-529.0526371
TCGTAAA1000.006045145429.0291442
GTGCGAT1000.00607916128.9958259
TATAACG2054.0422492E-728.2910165
GTTACGT1555.4336706E-528.1154561
CCGACTA2853.237801E-1027.9819984
GTCGAAG1100.00965901526.371053
Produced by FastQC (version 0.11.5)