FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1787_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1787_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618102
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG131450.8123715315845356No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC81280.5023169120364477No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT74350.45948895681483615No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG64580.39910957405651803No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC57030.3524499691614002No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG54890.3392245977076847No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG51340.31728531328680143No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA51220.3165437036725744No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG47410.2929975984208659No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT44670.27606417889601526No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA40310.24911902957909945No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA38630.23873649497992092No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT38200.23607906052894068No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC34650.21413977610805746No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA34020.2102463256333655No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA33870.20931931361558173No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG32220.19912218141995994No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT32110.19844237260691847No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA31200.1928184996990301No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC30780.19022286604923547No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT29700.18354837952119213No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT29020.17934592504057223No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA28410.17557607616825144No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC28330.17508166975876677No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA27120.16760377281531078No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT27060.16723296800819726No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG26530.16395752554536117No Hit
ATACTGAGGACCCAGATGAAGGAACAGCAGGAGTAGAGTCTGCAGTATTG25960.16043487987778274No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT25680.1587044574445863No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT25320.1564796286019052No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC24700.15264797892839882No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC24520.15153556450705827No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG24390.15073215409164564No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG24110.14900173165844924No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG24050.1486309268513357No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC23710.14652969961102574No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT23440.14486107797901493No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG23310.14405766756360228No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC22980.14201824112447795No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG22700.1402878186912815No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC21770.13454034418102198No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG21240.13126490171818586No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG21190.13095589771225793No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA21010.12984348329091738No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT20960.12953447928498946No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA20870.12897827207431917No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA20680.12780405685179302No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC20470.1265062400268957No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT20310.1255174272079263No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA20020.1237252039735443No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC19940.12323079756405964No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT19690.12168577753441996No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA19020.11754512385498567No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT18980.11729792065024332No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA18900.11680351424075862No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC18840.11643270943364509No Hit
TGCTAGTACTACTTGAAAATCAAAAAACACTCGATGAGCATGATGCGAAC18810.11624730703008834No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC18600.11494949020519102No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG18600.11494949020519102No Hit
CCTTACTAGCAAAATCTGTGTTCAACAGCCTATATTCATCTCCACAACTC18560.11470228700044868No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC18510.11439328299452074No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA18470.1141460797897784No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA18380.1135898725791081No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG18320.11321906777199461No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT18210.11253925895895316No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC18170.11229205575421079No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG18090.11179764934472611No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA17990.11117964133287024No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC17980.11111784053168465No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT17930.11080883652575671No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT17780.10988182450797292No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT17730.10957282050204499No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT17460.10790419887003413No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG17420.1076569956652918No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA17400.10753339406292065No Hit
TTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTGT17320.10703898765343593No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC17180.10617377643683773No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC16960.10481415881075481No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG16900.1044433540036413No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG16700.10320733797992956No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC16550.10228032596214577No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGCA100.0070983353144.95168145
GCAGGTA12800.0116.24342
GGTACTG13900.0105.872185
CAGGTAC15250.097.498843
GTACTGA16700.092.0166246
GACTTAA800.090.594795145
AGTAGAA10300.083.918331
AGGTACT18350.082.1930854
ACTGAGG4000.079.782583
AACACTG5950.074.321335
TTTCGTT608.053394E-872.47584145
CCGACGC1150.069.32471145
AAAAGCC1800.064.516662
CTGCCGC3000.062.81239145
TCTGACC1051.6370905E-1162.122147145
TTTTTGG15450.062.0572551
TTTGGAC15750.060.7867363
CTGATCC25300.059.584889
TATGGAT38600.056.0342252
GACAGTA17100.055.9462627
Produced by FastQC (version 0.11.5)