FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34315222
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG849660.24760440133536074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC89900.033.542853
GGATAGC102050.030.4586185
TATACTG165000.026.2002455
ACTGATC148850.024.8119968
AGTGCGT196250.023.9880968
GCAGGTA112250.023.3015272
GTATGGA175250.022.2914351
GAGTGCG210950.022.2825937
TGGATAG145350.021.4834564
CAGGTAC126100.021.3068873
CCTTATA152150.020.7606032
TATGGAT166100.020.221712
GTGCGTG239400.020.1416879
TTGGACA131700.020.129174
TCTCCGC73850.019.824022145
GACAGTA114550.019.7065857
ACGCGCC33250.019.336198
GTTACGT19500.019.1147351
ATACTGG182900.018.9089266
TATAGCG14850.018.2871842
Produced by FastQC (version 0.11.5)