FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_pDZ_PB2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_pDZ_PB2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882966
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT63140.3353220398031616No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC61980.32916154619892235No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA58080.3084495418398421No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG56800.3016517557937849No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA54640.29018049184106354No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC54270.2882155068121251No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT49930.2651667634997127No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA47990.2548638690236573No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT47910.2544390073957788No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT45250.24031235826881633No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT44730.23755075768760564No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG41480.2202907540550387No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT40810.2167325379215557No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT38730.20568613559671284No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG38400.20393358138171375No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC35270.18731087019096468No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC35260.18725776248747986No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT34990.18582385449338967No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA34280.18205320754596738No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT33690.1789198530403629No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT33050.17552096001733436No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA31420.1668644043493085No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT31350.16649265042491473No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC31270.16606778879703618No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC31140.1653773886517335No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT30900.16410280376809777No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT30690.16298754199491652No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA30410.16150052629734152No Hit
ATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTC30390.1613943108903719No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG30050.159588648971888No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT29980.15921689504749423No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT29710.15778298705340404No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC29660.15751744853597993No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG29640.1574112331290103No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC29570.15703947920461656No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT29050.15427787862340583No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC28610.1519411396700737No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC28260.15008237004810496No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA27930.14832981583310587No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC27710.14716144635643977No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT27270.14482470740310766No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT27130.14408119955432014No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT26150.13887664461280766No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA26090.13855799839189872No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA25950.13781449054311123No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT25580.13584950551417285No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT25370.1347342437409916No Hit
GATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCT24900.132238181677205No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT24790.13165399693887198No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA24550.13037941205523626No Hit
TTCCTATTCCACCATGTCACAGCCAGAGGTGATACCATCACTCGGTCTGA24540.13032630435175144No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGT24370.12942347339250948No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC24320.12915793487508537No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT24170.12836131932281306No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT23930.12708673443917734No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGAT23700.12586525725902645No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT23660.12565282644508716No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT23600.12533418022417825No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG23300.12374094911963361No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA23050.12241325653251306No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT22910.12166974868372557No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT22790.1210324562419077No Hit
TTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACTCT22770.12092624083493808No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG22460.11927990202690861No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA22130.1175273478119095No Hit
ATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCCCAG22040.11704937848054611No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT22010.11689005537009166No Hit
GAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAAC21930.11646519374221309No Hit
GTATGATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAAT21850.11604033211433451No Hit
TCTCCCAACCATTGTGAACTCTTCATATCCCTCATGCACTCTTATCTTCA21760.1155623627829711No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT21750.1155092550794863No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA21680.11513750115509254No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC21610.11476574723069881No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT21570.1145533164167595No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT21490.11412845478888094No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA21490.11412845478888094No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA21470.11402223938191129No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT21380.11354427005054792No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT21200.11258833138782112No Hit
GTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGA21070.11189793124251844No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT20960.1113137465041854No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT20880.11088888487630684No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA20830.11062334635888274No Hit
TTATTGGAGATGTGCCACAGCACACAGATTGGTGGAATTAGGATGGTAGA20710.1099860539170649No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT20680.10982673080661043No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT20660.10972051539964078No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGTCAATG20590.10934876147524702No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC20570.10924254606827738No Hit
TCCCAGAACCCTACAATGCTATACAATAAAATGGAATTTGAACCATTTCA20570.10924254606827738No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC20560.10918943836479257No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT20500.10887079214388365No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT20480.10876457673691399No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA20290.1077555303707024No Hit
CTCCAATAAAGATGCTAGTGGATCTGCTGATACTGCAGCTCTTCTCACTA20180.10717134563236937No Hit
ATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTTTGGTG20160.10706513022539972No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGAGATGTGCCACAGCACACA20140.10695891481843006No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG19880.10557811452782472No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA19850.10541879141737025No Hit
AGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTG19750.10488771438252203No Hit
ACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTAT19010.10095774432464526No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA253.7081918E-8145.029455
GTAGTCC100.0070915543144.99864145
GCACACC1200.096.665764145
GCACACT406.119917E-790.624159
AGGTGTG406.119917E-790.62415145
GAGATGG1250.087.0130541
TCGATGG259.388394E-486.99918145
TTTCGCG801.8189894E-1281.56174145
AGATGGG1350.080.571922
AACCGGC406.665023E-572.49932145
CGAGGGC554.0632203E-665.90848145
TATATAT6450.065.207052
CACGAGC1450.064.9994145
GTATATA6500.064.702011
GAGTTAA451.1941862E-464.44385145
GATGGGG1700.063.983583
GAGAGTG1850.058.783239
ACACATA1500.057.99946145
GTGTTTA502.0107953E-457.999454145
GGGGAGA1900.057.248476
Produced by FastQC (version 0.11.5)