FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_pDZPB2_nonamp_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_pDZPB2_nonamp_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49816
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG1130.22683475188694396No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC960.19270916974466035No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT950.19070178255982012No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT930.18668700819013972No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT900.18066484663561908No Hit
CCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCC870.17464268508109845No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG820.16460574915689738No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA770.15456881323269633No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT730.14653926449333549No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT680.1365023285691344No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC670.13449494138429421No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG660.132487554199454No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT650.1304801670146138No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA630.12646539264493337No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG620.12445800546009313No Hit
CTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT620.12445800546009313No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC610.12245061827525293No Hit
GCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA590.1184358439055725No Hit
CCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTC580.1164284567207323No Hit
CTCCAGGATTGCAAAATTTCTCCTTTGATGGTTGCATACATGTTGGAGAG580.1164284567207323No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA580.1164284567207323No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT570.11442106953589207No Hit
GTACCCGGGCATGCTCGAGCTAGCAGATCTTTTTCCCTCTGCCAAAAATT550.11040629516621166No Hit
ATCCTGGAGTTCTTCTTTCTTCTCTTTGGTTATCGTTAGTTGCGATTCCG550.11040629516621166No Hit
CACTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCT540.10839890798137145No Hit
GTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCA530.10639152079653125No Hit
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG530.10639152079653125No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG530.10639152079653125No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT530.10639152079653125No Hit
AGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT520.10438413361169101No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC520.10438413361169101No Hit
GGACTATGGTTGCTGACTAATTGAGATGCGGATCCGCTGGCACGACAGGT520.10438413361169101No Hit
CTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT520.10438413361169101No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA520.10438413361169101No Hit
GTCTAGAATTGACCTGCATGGTACCCGGGCATGCTCGAGCTAGCAGATCT510.10237674642685081No Hit
CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG510.10237674642685081No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC510.10237674642685081No Hit
ATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAGTGCTT510.10237674642685081No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT510.10237674642685081No Hit
GTTTAAAAACGACCTTGTTTCTACTAATAACCCGGCGGCCCAAAATGCCG510.10237674642685081No Hit
GTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCTGAAAATGT510.10237674642685081No Hit
GCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT500.1003693592420106No Hit
GAATATAATTGACCTGCTTTCGCTCCCCCCCAACTTCGGAGGTCGACCAG500.1003693592420106No Hit
CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCAC500.1003693592420106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAC100.0070707877145.0145
CACCTCG100.0070707877145.01
ACCTCGA100.0070707877145.02
CGACCAT100.0070707877145.06
GCCCACC256.404269E-6116.0145
CGCTGAG300.001924921972.5145
AGGGGAC350.003546641262.1428573
GACGGCG350.003546641262.1428577
ACGGCGG350.003546641262.1428578
GGATGTC400.006017312454.3757
CCCAGGT400.006017312454.3752
AAGGGGA400.006017312454.3752
ACCCAGG400.006017312454.3751
CAGGTGG400.006017312454.3754
CCAGGTG400.006017312454.3753
TAAGGAT400.006017312454.3754
GCCTAAG450.0095858548.3333361
GATGTCT450.0095858548.3333368
CTAAGGA450.0095858548.3333363
ATGTCTA450.0095858548.3333369
Produced by FastQC (version 0.11.5)