FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_Negative_Control_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_Negative_Control_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCATTGACGATGAGTAAGTTATTGTCAGTTTCTCTGTTCCCTGTGTTT15.263157894736842No Hit
CACAATCACATATGGAGCAGAATCTTGTAATTATGTCGAAAAAAGAAGCC15.263157894736842No Hit
GGGGTAAGAGGCAAGTCTGGCTCTAGGCTGGTCCCACTGTTGGGAAGCAC15.263157894736842No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC15.263157894736842No Hit
CTGAGAATACTGCAGTGGTTTCCCGTGATAGGAAATCTGAGGGGCACACC15.263157894736842No Hit
GATATCAGTGATGTTTAAGTTTCAGATGTTTTTTTGGGAAATTACTGATT15.263157894736842No Hit
AGCAGGAGGCCAGCAGCGGGGAGAAAGATTGGAGACGGTGGAATGGCCAT15.263157894736842No Hit
TTTTACACCTTTCTCACAAGTCATGAATTAAGGTTCCTGGAATACAGACC15.263157894736842No Hit
GGGGATAGACCTCTCTTTATTTTTTAATTTAGTCTTTTATTCTTTTGAGA15.263157894736842No Hit
GGTAAAATCCTGAACCAGAAATATTCCCCCTTCTGCAGAGAAAGAGGCAG15.263157894736842No Hit
AATATACTCCTCTCAAGTGCCCCTAGAATGTTCTCAACCTTAGGTCATAT15.263157894736842No Hit
TGATTAGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTGTGAGTTTC15.263157894736842No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA15.263157894736842No Hit
TCCATGATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGG15.263157894736842No Hit
CTTTTAAGACATTTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGG15.263157894736842No Hit
GCATTTGACTCAAGGAACATGCTGGGAACAGATGTATACTCCAGGAGGGG15.263157894736842No Hit
GGTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACG15.263157894736842No Hit
AGACCTCCCAAGTATCTGTGATGAACATAAACATAAAAATCCTCACCCAA15.263157894736842No Hit
CTCCCACATCATTGACGATGAGTAAGTTATTGTCAGTTTCTCTGTTCCCT15.263157894736842No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)