FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_Chang_07_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_Chang_07_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6124
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT380.6205094709340301No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT320.5225342913128674No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT250.4082299150881777No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG180.2939255388634879No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA180.2939255388634879No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT170.2775963422599608No Hit
TTTGAGAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC160.2612671456564337No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT150.24493794905290658No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA140.22860875244937948No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA130.2122795558458524No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT120.1959503592423253No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT120.1959503592423253No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT120.1959503592423253No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT110.17962116263879815No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA110.17962116263879815No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA110.17962116263879815No Hit
GCTTGTTTCTGTCTCCTCTTGTTCCATTGTAGTATCCCCCTGGTTGGGCT100.16329196603527107No Hit
GCTCCCAAATGCTTCAAATGCATCCCATTGGAACATGTCCTCTTTTCCTA100.16329196603527107No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG100.16329196603527107No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG100.16329196603527107No Hit
AGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGAA100.16329196603527107No Hit
CTTTATGAACTGGTCAGTTTTCATAACCTCCTGGTCTCTCATTTGTTTGA90.14696276943174394No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT90.14696276943174394No Hit
GTACTGACCAGCCATCTTCTGAGGAATTATGCTCCCAAATGCTTCAAATG90.14696276943174394No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG90.14696276943174394No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT90.14696276943174394No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATAGACAGATGGAG90.14696276943174394No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT90.14696276943174394No Hit
TTCTATGGTTTTATTGCTGGCAAATCCGCATGTGCATTCCTCAGTGTGTT80.13063357282821686No Hit
CCTTTGATTCACTGAATATTACTGCTGCATCTTTAAATGACGATGGATTG80.13063357282821686No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA80.13063357282821686No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG80.13063357282821686No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC80.13063357282821686No Hit
CACGTGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG80.13063357282821686No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG80.13063357282821686No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA80.13063357282821686No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG80.13063357282821686No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA80.13063357282821686No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA80.13063357282821686No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA80.13063357282821686No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA80.13063357282821686No Hit
TAGTTAGGACTTCTGTTTGTGGATTATAGGAAAATAGAGGGGACCCTTTC70.11430437622468974No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA70.11430437622468974No Hit
CCCTTAGTATCAGTCACACCTAAAAAGTTAACATGGGAGGACCTAAGACC70.11430437622468974No Hit
TTTGAAAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC70.11430437622468974No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCTGAGTCCACTCTTGGATC70.11430437622468974No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC70.11430437622468974No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA70.11430437622468974No Hit
GTCCTTGAGAGTGTTGGTAAACGGAACATTCCTCAAACACCCCAATGGAC70.11430437622468974No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA70.11430437622468974No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT70.11430437622468974No Hit
AACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCA70.11430437622468974No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG70.11430437622468974No Hit
GTATTAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTGGAGA70.11430437622468974No Hit
CATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTG70.11430437622468974No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA70.11430437622468974No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG70.11430437622468974No Hit
GCTCTTGCAAATCCACTGTACTGACCAGCCATCTTCTGAGGAATTATGCT70.11430437622468974No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA70.11430437622468974No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAGCA70.11430437622468974No Hit
CCTCCATGTTGTCAGAGAGTACTTCCTTCATTGTTTCTTTGGCCTGGATT70.11430437622468974No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT70.11430437622468974No Hit
TGATAAGGCTCCCCATTGCTCCTTAATTTTGGTGGTGAGAAACAAAAAGG70.11430437622468974No Hit
GTATTAGTAGTTGAAAACTTGGCTAAAAATATATGTGAAAATCTAGAACA70.11430437622468974No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAG100.006920232145.01
CCAAGAA100.006920232145.0145
CTCCGAA100.006920232145.06
GGGGGGG4405.456968E-129.886364145
Produced by FastQC (version 0.11.5)