FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_Chang_06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_Chang_06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36408
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC1610.44221050318611294No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT700.19226543616787517No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG680.18677213799165018No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT650.17853219072731266No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA570.15655899802241266No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCACTTGAAAGAAAGCTGAA530.14557240166996266No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT510.14007910349373764No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT510.14007910349373764No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT470.12909250714128764No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC470.12909250714128764No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA470.12909250714128764No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA450.12359920896506263No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA440.12085255987695012No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATACTAAGAACAC440.12085255987695012No Hit
TATGATAACTGATGGCTCAGCTTCAGGTGTTAGTGAATGCAGATTTCTTA440.12085255987695012No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC430.11810591078883761No Hit
GTACTGACCAGCCATCTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG430.11810591078883761No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAAGAGG430.11810591078883761No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA430.11810591078883761No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGCAAACAAG420.11535926170072512No Hit
CTTTATGAACTGGTCAGTTTTCATAACCTCTTGGTCTCTCATTTGTTTGA410.11261261261261261No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG400.10986596352450011No Hit
CTATATGTGTCAGCTTTATTATCATACTTACTATATTCGGATATATTGCT400.10986596352450011No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC390.10711931443638761No Hit
TACTGACACATACCATTCCTATGCAAACAAAATACTAAGAACACAAGAAA380.1043726653482751No Hit
AGTAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAAT370.10162601626016261No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT370.10162601626016261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG100.0070630163145.01
GTAACAA550.0118.636364
TAACAAG650.0100.384615
TAGAAAC259.313726E-487.02
CCTTAAG259.313726E-487.01
TAGTAAC851.8189894E-1276.76472
GTAGAAC300.001920739372.53
TAAGAGC300.001920739372.54
CAGAGTG300.001920739372.59
AGTAACA955.456968E-1268.684213
TGTAGTG350.003538963662.1428575
TTAAGAG350.003538963662.1428573
ACAAGAG1205.638867E-1154.3757
AACAAGA1301.2369128E-1050.1923076
GTAGTAA1752.2955646E-937.2857131
CAAGAGC1601.8122864E-631.718758
GAGCATT1402.6752734E-531.0714284
TGACCAG1306.362811E-427.8846155
GCATTTT1658.201941E-526.3636366
AGAGCAT1500.001466847524.1666683
Produced by FastQC (version 0.11.5)