FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_Blank_Space_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_Blank_Space_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733840
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT181111.044560051677202No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC135440.7811562773958382No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA98620.5687952752272413No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG93280.5379965856134361No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA90340.5210400036912287No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA88180.5085821067687907No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG80050.46169196696350295No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT78020.4499838508743598No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA71180.41053384395330594No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA69850.40286300927421215No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC66640.3843491902367No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA63690.3673349328657777No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC55770.32165597748350483No Hit
GGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGA53950.31115904581737647No Hit
GTTTAGACCTGTACCCAGCAACTGATTACACAGTGAATGTCACTCTCCTG52950.30539150094587736No Hit
ACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGTGGTTGGAA51110.294779218382319No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA50240.2897614543441148No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA50030.2885502699211No Hit
TTACCATACAGATGACTGTGCTTTTTAAAGTAACAGACTCTGAACTCCAA49640.2863009274212154No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG49570.2858971992802104No Hit
TATTAAAGGTCATTTCCTGGGCAAATTCCTTCTGATACCATCTCTGGCCC48450.2794375490241314No Hit
GCTTAACAGCTATCATTTTAGGATATAAAAAGCGGTTTTCTTTTTATCTT46810.26997877543487286No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC46490.26813316107599316No Hit
GTCATATTAAAGGTCATTTCCTGGGCAAATTCCTTCTGATACCATCTCTG45800.2641535551146588No Hit
GTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGTGGTTGG45350.2615581599224842No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA44990.25948184376874456No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG44410.25613666774327504No Hit
GTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCTTG44240.25515618511512017No Hit
CCCCAGAAGTGTGCCTAGATCTGCACCGAGGCACCAACTACACCATGAGC44220.2550408342176902No Hit
TAATATGACTGCCCACAGCCAGGCTCCTGAGATGTGCCTGGACCTGCGTC43330.249907719282056No Hit
GATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATATTA42360.24431320075670188No Hit
GTAGAATAGTGGTTGGAAGGGGCTGGGGAAAGAGGAAAATGGGGAATTGT41790.2410257001799474No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG41460.2391224103723527No Hit
ACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGAGATAA41440.2390070594749227No Hit
AAGTAGAATAGTGGTTGGAAGGGGCTGGGGAAAGAGGAAAATGGGGAATT40440.2332395146034236No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC40000.230701794859964No Hit
TTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCT39760.22931758409080422No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT37570.2166866608222212No Hit
GTCTTTGTACTTCTCCATCTCAAGCAGGTTTCGTTAAATACTGAAATGTT36770.21207262492502194No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT35520.20486319383564805No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA35090.20238314954090345No Hit
AAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACT34200.19725003460526924No Hit
CTCTTAGAAAGGTAGTCAGGACAAAATACTAATGCGAGTTAAATGTGGCT33970.19592349928482444No Hit
CTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAA33950.19580814838739444No Hit
TTTCAGATCCACTTGATAAAAAAGGGCTATTTCCTTTATTCAGTTCTTTG33840.19517371845152953No Hit
TCTCAGGAGCCTGGCTGTGGGCAGTCATATTAAAGGTCATTTCCTGGGCA33600.19378950768236977No Hit
ACAGATGACTGTGCTTTTTAAAGTAACAGACTCTGAACTCCAATGTTGAT33420.19275134960549992No Hit
CTCCTGAGGCACTCACGACAAAATGACGATTACACATAACTGAGAAGCCG33310.19211691966963504No Hit
CCTCTGTATCAGCGGTCTTTGTACTTCTCCATCTCAAGCAGGTTTCGTTA32840.18940617358003045No Hit
CCTTTATTCAGTTCTTTGAACTCTTTTCCTCTGTTTCCTCATTTAATCAA32340.18652240114428092No Hit
GTACAAAGACCGCTGATACAGAGGAGATGTATTTATTCTACATTCGGGGC31620.18236976883680156No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA31600.18225441793937158No Hit
ATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTA31090.179312970054907No Hit
TAACTGAGAAGCCGTCATGAGGAACAATTCTTTTTAGGTCTCGAGTATGT31050.17908226826004706No Hit
CAGTAAAACAGATCATCGGTAACATTTCAGTATTTAACGAAACCTGCTTG30520.17602546947815254No Hit
ACACATAACTGAGAAGCCGTCATGAGGAACAATTCTTTTTAGGTCTCGAG30160.17394915332441285No Hit
GTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATCTCCAA29880.1723342407603931No Hit
ACTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACA29500.17014257370922345No Hit
ATACAGAGGAGATGTATTTATTCTACATTCGGGGCCAGAGATGGTATCAG29220.1685276611452037No Hit
CTCCTGGGATCTTCTGAGCAGCAGTCAGTGCAAATAACGATGACAACCGC29000.1672588012734739No Hit
GTTGTAGCACACACCTGAAGGGGGGGGGTATGAAAGGGTCCTGTGGTCGT28570.1647787569787293No Hit
GAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGTGGTTGGAAGGGGC28490.16431735338900938No Hit
GTATTGGGGTGAGTCACAGTTCTCAAAACAGAGAGTAAATCCTGTCACTT28400.16379827435057445No Hit
GGTCTAAACATACCACTGGAGCTCGCTCCCTTGTTGTGAAGTTAAATGAC28310.16327919531213952No Hit
GAATAAATACATCTCCTCTGTATCAGCGGTCTTTGTACTTCTCCATCTCA28300.16322151986342454No Hit
GTATTGGAGGGCACCAGACTGGCTCGATCAGTAGAGCGGGCAATGCTTGC28130.1622410372352697No Hit
GATCTGCACCGAGGCACCAACTACACCATGAGCATTTCCACCGCCCCTCC27790.16028007197896No Hit
CCTCCAAATAGCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAA27780.160222396530245No Hit
GGCTACAGCATGGATGAACTTGGAGATCATATGTGAAGTAAAATAAGCTA27730.15993401928667006No Hit
GTCCCACACCAACCAGGTGCTTGAAGCAGGCTGTGTGGAAGGGCAGGCAT27360.1578000276842154No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA27240.1571079222996355No Hit
CTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTA26880.1550316061458958No Hit
CCCCAGATTTGAGGAGCGACAATGAGAAGATGAGAGAAAAGTGAAATTGG26790.1545125271074609No Hit
GTATGTATACACCATGTTTTGTTTATCCATTCATCATCAGAGCACATTTG26560.15318599178701608No Hit
ACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAG26310.15174410556914134No Hit
AGTAGAAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTC26270.15151340377428135No Hit
GTATTTATTCTACATTCGGGGCCAGAGATGGTATCAGAAGGAATTTGCCC25730.14839892954367184No Hit
ATTTTAAATATCCGGAAGGAATGTAACCAGCGCTCACATACACGCTAGCC25260.14568818345406728No Hit
TCTGAAGACCAAGTAATATGCCATTGTATGTATACACCATGTTTTGTTTA25250.1456305080053523No Hit
TACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATA24980.14407327089004754No Hit
ATACTAATGCGAGTTAAATGTGGCTAGCGTGTATGTGAGCGCTGGTTACA24920.14372721819775758No Hit
CTGATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATAT24910.1436695427490426No Hit
GGATGGAGCCATAGCAGAATCCTCCATACCAAGAGCTTTTCAGATCCACT24800.14303511281317768No Hit
GTATCAGCGGTCTTTGTACTTCTCCATCTCAAGCAGGTTTCGTTAAATAC24630.14205463018502285No Hit
CTTCTAATTGTGGTAAAATACAATAACACAAAACTCACACCTTAATCATT24210.1396322613389932No Hit
GTGAATGTCACTCTCCTGGGATCTTCTGAGCAGCAGTCAGTGCAAATAAC24040.13865177871083836No Hit
GCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAATGGATAAACA23740.13692151524938864No Hit
TCATTTAACTTCACAACAAGGGAGCGAGCTCCAGTGGTATGTTTAGACCT23740.13692151524938864No Hit
GTTTTACTGCTGGTGCGGTTGTCATCGTTATTTGCACTGACTGCTGCTCA23250.13409541826235408No Hit
ACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTT23200.13380704101877913No Hit
CACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCAT23180.13369169012134915No Hit
GAATGTAACCAGCGCTCACATACACGCTAGCCACATTTAACTCGCATTAG23050.13294190928805424No Hit
GAATAAGGTTCTGTTTTGTTAAGGTGGCACAGGAGGTCCTTTAAACCTAA22900.1320767775573294No Hit
TCCCAGGAGAGTGACATTCACTGTGTAATCAGTTGCTGGGTACAGGTCTA22630.13051954044202463No Hit
CCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCC22400.12919300512157986No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCTGAAGACAGATACTTAACTCT22390.12913532967286484No Hit
CCTTTAATATGACTGCCCACAGCCAGGCTCCTGAGATGTGCCTGGACCTG22320.12873160153185992No Hit
ATACAGATGACTGTGCTTTTTAAAGTAACAGACTCTGAACTCCAATGTTG22100.1274627416601301No Hit
CATACACGCTAGCCACATTTAACTCGCATTAGTATTTTGTCCTGACTACC22040.12711668896784017No Hit
ATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTT21910.12636690813454526No Hit
GGTCAAAGGTGGTACCTGAATAATTTTTATCATGCAACGTCATTTAACTT21690.1250980482628155No Hit
CTACAGGTATGGCCACAGTTGACAGAGTATTGGGGTGAGTCACAGTTCTC21650.12486734646795553No Hit
GAATAAAGGAAATAGCCCTTTTTTATCAAGTGGATCTGAAAAGCTCTTGG21600.12457896922438057No Hit
CATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGAATAGT21470.1238291883910857No Hit
CTCCTGAGATGTGCCTGGACCTGCGTCCGGGCACCAACTACAGCATCCGC21410.12348313569879575No Hit
ACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACA20960.12088774050662114No Hit
CATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGT20850.12025331057075625No Hit
TTCTAAGAGAGAAGACATGACAGGTACGACCACAGGACCCTTTCATACCC20820.12008028422461127No Hit
GTCATGTCTTCTCTCTTAGAAAGGTAGTCAGGACAAAATACTAATGCGAG20760.11973423153232132No Hit
CCTCCATACCAAGAGCTTTTCAGATCCACTTGATAAAAAAGGGCTATTTC20500.11823466986573156No Hit
CTCCTATTCTTCCACAAAGGATGGAGCCATAGCAGAATCCTCCATACCAA20370.11748488903243667No Hit
GTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTC20330.11725418723757672No Hit
CTTTTTATCTTTGTGTAAGGTAAGCTTCCAAGTTTTGGTGGAAATTCCAG20210.11656208185299682No Hit
TTTGAAATGTGCTCCTGCTTAACAGCTATCATTTTAGGATATAAAAAGCG20060.11569695012227195No Hit
GAATAGTGGTTGGAAGGGGCTGGGGAAAGAGGAAAATGGGGAATTGTTCA19890.11471646749411711No Hit
GTAATATGCCATTGTATGTATACACCATGTTTTGTTTATCCATTCATCAT19860.11454344114797213No Hit
GCATATTACTTGGTCTTCAGATGGATGGAAATCCTGACACGGGCTACAGC19700.11362063396853227No Hit
GTGCAAATAACGATGACAACCGCACCAGCAGTAAAACAGATCATCGGTAA19580.11292852858395237No Hit
GCCAAGCACTGTTCCCTGAGAATAACGGACTCCTGAGGCACTCACGACAA19040.10981405435334286No Hit
CTCCATGCCGGCCATCATCGGGTTCCAGACAGCTGTTTCAAGTTCTGGGT19010.1096410280071979No Hit
CTTCTGAGCAGCAGTCAGTGCAAATAACGATGACAACCGCACCAGCAGTA18960.10935265076362294No Hit
GTAAAATACAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACG18880.108891247173903No Hit
GCTCTCTACACTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGTTTTGT18840.10866054537904304No Hit
GTGTAAGGTAAGCTTCCAAGTTTTGGTGGAAATTCCAGCGTGATTCCATT18810.10848751903289808No Hit
AAATACATCTCCTCTGTATCAGCGGTCTTTGTACTTCTCCATCTCAAGCA18530.10687260646887833No Hit
GTAAAACAGATCATCGGTAACATTTCAGTATTTAACGAAACCTGCTTGAG18510.10675725557144834No Hit
GGCTAACATTACCATACAGATGACTGTGCTTTTTAAAGTAACAGACTCTG18460.10646887832787338No Hit
GTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAG18430.10629585198172842No Hit
GTTCAAGGACAACAGAGTTTCACAAGATGAAAAAGTTTTGGAGGTTGTAC18370.10594979928943847No Hit
TACCTGTCATGTCTTCTCTCTTAGAAAGGTAGTCAGGACAAAATACTAAT18370.10594979928943847No Hit
GTTCCAGACAGCTGTTTCAAGTTCTGGGTCAAAGGTGGTACCTGAATAAT18120.10450791307156371No Hit
AATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACATACAA18010.1038734831356988No Hit
AAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTC17910.10329672864854889No Hit
CTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGT17790.10260462326396899No Hit
GCCCACAGCCAGGCTCCTGAGATGTGCCTGGACCTGCGTCCGGGCACCAA17670.1019125178793891No Hit
TGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTAC17590.10145111428966917No Hit
GATTACACAGTGAATGTCACTCTCCTGGGATCTTCTGAGCAGCAGTCAGT17580.1013934388409542No Hit
GATTTGAGGAGCGACAATGAGAAGATGAGAGAAAAGTGAAATTGGGAGGA17490.10087435980251926No Hit
CCCCTGCTCTCTACACTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGT17470.10075900890508928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8150.0126.332811
GCGAAAG10100.0100.506022
ACGATCC451.0921212E-896.66607145
GAGCGAA700.093.2244641
CGAAAGC11050.091.8652343
GCCTATG259.3881466E-486.999466145
CAAGCCG259.3881466E-486.999466145
CTACAGG3850.086.632841
CCAGAAG8150.086.297773
CAGGTAT3950.084.43964
AGACGCG1550.084.19303145
AAGTGTG8650.082.9763647
TTAGTAG1350.080.5643542
TCTCCGC5600.080.267365145
ATCTCCC3350.075.745804145
AGTGTGC9450.075.184728
CCACGAC1750.074.57097145
GTAGAAA7700.074.391242
TCCGCAA300.001936030172.49955145
GTGTGCC10200.068.945659
Produced by FastQC (version 0.11.5)