FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_Blank_Control_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_Blank_Control_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGGCAGGCAATTGCTTGGGGCCAGATGTTCAAGACCAGCCAGGGA24.0No Hit
ACCCTCCTTAACATCCATGGGAACACGGGTGCCCTTGTCGTCGACGCGAC12.0No Hit
GGTCAGGTAAAGCTTTATTAAATGGTAAACATTTTTAACAGTGGAGAAAT12.0No Hit
CTGACGTGTGACGTACAAGCATTGTTTCTGGCACTGTTGCCGGGGATATA12.0No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT12.0No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG12.0No Hit
CTACTAATAGAGGGCACAGCCTCATTGCGTCCAGGGATGATGATGGGCAT12.0No Hit
CATCACTTCATTGTCCTAAATCAGTCGCGTGGCTGCGGCGTCGAGGAGGT12.0No Hit
AAAGAGGGATCGCCAAATGTTTGGAACAGTTTCTGTAGAAAAGGACTAAG12.0No Hit
GGTGGCAAGGGTAGAGTTTCAGGCCACCAGCTGGAAAATCCCGGTTCCAA12.0No Hit
ACCTAAACCGAATAAAGAGTTGGACACTCAACCTACTCAGCCACCAAGGT12.0No Hit
GAGTTTGGCCTTGCTCATTCCTTTCAGGGATCATCTCCATTATTCTTTTG12.0No Hit
GACCTTAAGTGAAAGACTAGCTGAGTTTAAACGAGATAAAGTAGCTAAAG12.0No Hit
GTTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATTTTAGTAGGTTGT12.0No Hit
GTGTATGTTTTCAGATTGAGTATCTCTTTTAACTCTAGACTCAACTGAGC12.0No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT12.0No Hit
GGCCATGGTGTCTAGGGCCCGAATTGATGCACGGATTGACTTCGAGTCTG12.0No Hit
GTTAAATACAATGAAGGATAAAATACGAGTATAACAATGAAAATTTTTAA12.0No Hit
AAGAAAAACTAACAAACAGAAAGGAATAGCATCAGCATCAACAAAAAGGA12.0No Hit
GCCAGAAGCTCCCAGGAAATCAAACCTTCCCACCATAGCACCAGGCCCGC12.0No Hit
AGATTATAGAGTAGATGGTTCCCAAACTTGGACATATAGAGATATTAATA12.0No Hit
GTTTTCTAGGCTGTTGAACACAGATTTTGCTAGTGCATTTGGATCACCAG12.0No Hit
TCCCAGTAGTGTGCCCCCACTTTCCATAGCTGGATAATGTATTCATCACT12.0No Hit
TCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGAC12.0No Hit
GATATGCTTGGAGCCATCCTAGGGAAAAAAAAAACAACACGAAGATCAGA12.0No Hit
ATGCTCTCCCGGTCACTCTTAAACTTGCGCGCTTCTCCTTGTTCAGATCT12.0No Hit
CACAGAGCACTGACTGGTGCATTTACAATCCTTTAGCTAGACAGAAAAGT12.0No Hit
GCCCACGAACCCAATTGGTTGTTCAGTAGAATGTTGTTTAGTCTCTGTGT12.0No Hit
TGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATA12.0No Hit
ACTTGGGGCTCAATCCCAGGATCGAGCTCATGCCCCGCGCCAAAGGCAGA12.0No Hit
ATTTAAGTCCTAATTTGCGAAAATTCTAGTCTTATCGAATTTATTGACTT12.0No Hit
CGAATATACCCGCGCCGTTGCCGGCACATCGCCGAGCCTCCCGCCATCAA12.0No Hit
AATAAGAACACATTTGCACAACTTCTGTTAACAACTTCGCTTCCAAATCT12.0No Hit
GCCTGAAGGACTGTGCAGAATTAAAATGGGGGAAGGCAGTAAGAGCAGGG12.0No Hit
AGCGAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACCCCCCACC12.0No Hit
CTCTTGTCACCCAGGCCAGAGTGCAATGGCGCAATCTCAACTCACTGCAA12.0No Hit
CTTTGGATCGTTTCAACGACGCCAAGCGCGCCGAAGTCAGAGACCGCGTT12.0No Hit
GAAATGGATGATGGCGATGAAATACCCGATCACAGCTGACAAAAGAATAA12.0No Hit
CTTGAGCTCCTGGGCTGCTAACACATCAGAGAGAAAGGAAGGTGTCATTT12.0No Hit
AAATTATACAATACGTGAAGAGGTATTATCCAGTAATATTATTTTCAGGG12.0No Hit
AAATAGAGATCACTTAGAATAGTACCTATGAGAGTAAGCCCTCAAAAAAT12.0No Hit
GAGCAGAAAACTGGAAACTCTAAAAAGCAGAGCACCTCTCCTCCTCCAAA12.0No Hit
ATACATAATACGTTCTGTCCAGTTTTTTTGTGAGTCAAAAGAAATGTATT12.0No Hit
GGGTTGTATGTTCTGTCTATCTACAGTGTGCCCAGCATTGTAGACCCCAC12.0No Hit
GTATATATGAGTAAACTTGGTCTGAAAGTTACCAATGCTTAATAAGTGCG12.0No Hit
AAATTGGAGTATAAGCTACAATTTGTATAGTAAAGAGAAGAAAAAATATA12.0No Hit
AGCATACAGTGTTGCTGGACACATATCTTGGCTGGCAGCTGGCCAGGCAA12.0No Hit
GGCTTAACCCACTGAGCTACCAAGGCGCCCCTAAGTTTAGAACTCTTATC12.0No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC12.0No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATT50.0145.06
TTCATTG50.0145.07
CATTGTC50.0145.09
CATCACT50.0145.01
ACATTCA50.0145.0145
ACTTCAT50.0145.05
CACTTCA50.0145.04
ATCACTT50.0145.02
TCATTGT50.0145.08
TCACTTC100.072.53
Produced by FastQC (version 0.11.5)