FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_AHK1073_99_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_AHK1073_99_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100794
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA9040.8968787824672104No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT6230.6180923467666726No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG6090.6042026311089946No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG6050.6002341409210865No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA5490.5446752782903744No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG5390.5347540528206044No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT3760.37303807766335295No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT3330.33037680814334186No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT3210.3184713375796179No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC3080.30557374446891683No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT3080.30557374446891683No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC3050.3025973768279858No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG3020.29962100918705475No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG2980.29565251899914674No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT2770.2748179455126297No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2760.2738258229656527No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG2740.2718415778716987No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT2740.2718415778716987No Hit
ATACTGAGGACCCAGATGAAGGAACAGCAGGAGTAGAGTCTGCAGTATTG2680.2658888425898367No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC2670.2648967200428597No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA2640.2619203524019287No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC2410.23910153382145763No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT2410.23910153382145763No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA2320.2301724308986646No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC2290.2271960632577336No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC2250.2232275730698256No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG2210.2192590828819176No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG2110.20933785741214755No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT2080.20636148977121654No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG2030.2014008770363315No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC1950.1934638966605155No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA1930.19147965156656152No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA1870.1855269162846995No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA1870.1855269162846995No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA1850.1835426711907455No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC1770.17560569081492947No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA1710.16965295553306745No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT1630.16171597515725142No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA1600.15873960751632044No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC1600.15873960751632044No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA1600.15873960751632044No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT1570.1557632398753894No Hit
GGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA1560.1547711173284124No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1550.1537789947814354No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC1530.1517947496874814No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC1480.1468341369525964No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG1450.14385776931166538No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA1440.14286564676468838No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA1430.14187352421771135No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG1420.14088140167073437No Hit
TCGTAAGATTGCTCAGTTCATTGATGCTTAATGCTGGTCCATATCTTTTG1410.13988927912375737No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG1340.13294442129491835No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT1320.13096017620096434No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA1320.13096017620096434No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC1320.13096017620096434No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA1320.13096017620096434No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA1280.12699168601305633No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1260.12500744091910232No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG1250.12401531837212532No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT1250.12401531837212532No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA1240.12302319582514831No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC1210.12004682818421732No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC1190.11806258309026332No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG1180.11707046054328629No Hit
CTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATC1180.11707046054328629No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA1160.1150862154493323No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG1160.1150862154493323No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG1140.11310197035537829No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG1130.1121098478084013No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA1120.1111177252614243No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC1120.1111177252614243No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA1110.1101256027144473No Hit
ATATGAAATAATTGATCATGAATTCAGTGAGGTTGAAACTAGACTCAATA1110.1101256027144473No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA1100.10913348016747028No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT1100.10913348016747028No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC1090.10814135762049328No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG1090.10814135762049328No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT1060.10516498997956228No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA1060.10516498997956228No Hit
ATTATAGGTCCCGTTCCGAATTGTTTCCATGCACTGATCATCACATTTAT1060.10516498997956228No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA1060.10516498997956228No Hit
CATTTATGGTATAGCTCGAAACAGCCTTTCCCATCTTCCATAGCATTGGA1040.10318074488560827No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA1030.10218862233863128No Hit
GATATGGACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG1010.10020437724467726No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC100.007081469145.03
TCCCACG100.007081469145.02
CCACGAA100.007081469145.04
TGCGTAG100.007081469145.0145
AGCGAAA700.0113.928571
AGAGAGC203.8558638E-4108.758
GCGAAAG750.0106.333332
GCAGGTA750.0106.333332
AACACTG352.7436E-7103.571435
GGTACTG800.099.68755
CGAAAGC800.099.68753
AGGTACT900.088.611114
GAGTAGG259.3620917E-487.01
ATAGCAG259.3620917E-487.03
GTAGGAT259.3620917E-487.03
GCAATAC259.3620917E-487.01
TAGGATG259.3620917E-487.04
AGTAGGA259.3620917E-487.02
GCGTACT259.3620917E-487.05
AGTAGAA1350.085.925921
Produced by FastQC (version 0.11.5)