FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2339_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2339_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502937
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG157820.6305392424979134No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG139970.5592230247904761No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG138920.5550279531606268No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC100120.40001006817191165No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG96430.38526738787272713No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA88830.3549030598852468No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG85330.34091948778574926No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA81770.32669619730740324No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA73400.2932554834580335No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT70340.2810298461367585No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT65810.2629311085336946No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA65590.26205214114458336No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA64280.2568182898730571No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA63360.25314260806404637No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC59330.2370415236180535No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC59120.23620250929208367No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA58910.2353634949661138No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT55050.21994161259352515No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG53770.21482762051142318No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA52200.20855498959822003No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA49600.19816719318145043No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC48970.19565015020354087No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT47300.1889779886589235No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG46320.18506258847106422No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT45990.18374413738739728No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG45460.18162662504090193No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA43560.17403554304403188No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC42790.1709591571821424No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG42640.1703598612350211No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG41900.1674033345625559No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT39380.1573351626509177No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT39270.15689567895636206No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG37990.15178168687426014No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA37740.15078286029572457No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA37720.15070295416944174No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG37290.14898497245436063No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT37180.14854548875980497No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT35720.14271234154115744No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT35590.14219295172031898No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG35260.14087450063665208No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG33790.1350014003548631No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG33550.134042526839469No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTATTTTCAG33480.13376285539747906No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG33310.13308365332407487No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG32820.13112595323014523No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG32540.13000726746218544No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT32290.12900844088364988No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC31810.12709069385286167No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA31670.12653135096888174No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC30820.12313534060186093No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG30750.12285566915987098No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA30610.12229632627589108No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT30330.12117764050793128No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT29920.11953956491913299No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT29620.11834097302489036No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC29260.11690266275179918No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT29040.11602369536268792No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA28650.11446552590017249No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG28220.11274754418509135No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG28140.11242791967995998No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT27800.11106951553315166No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA27760.11090970328058597No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG26810.10711416228215093No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG26530.10599547651419113No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA26460.10571580507220117No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA26220.10475693155680707No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA25760.10291909065230168No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA25520.10196021713690757No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT25470.10176045182120047No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA25330.10120110893722055No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC25270.10096139055837203No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA25210.10072167217952349No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA25040.10004247010611933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA27600.088.282492
GGTACTG28100.087.229525
CAGGTAC30600.079.8643343
GTACTGA34550.072.414276
AGTAGAA31150.066.348521
ACTGATC39600.065.545898
AGCAGGT38550.063.582371
TTTGGAC36350.062.2435873
GCGAAAG7400.060.7581252
AGGTACT42750.060.219434
TTGGACA38950.059.0195964
TACTGAT43800.057.2742777
CTGATCC43350.057.0325669
GACAGTA40850.055.1983347
GTCTACG400.006046082454.388321
TTTTTGG41750.053.4981351
ACAAGGC21000.052.8238148
TGGACAG45700.051.0966875
CACGAGC6150.050.69031145
CCACGAC4050.050.12272145
Produced by FastQC (version 0.11.5)