FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAATAGCTAAAAGGTTATTCAACCCAAATGTGCTCTGATGATGAAT10.9615384615384616No Hit
CCACAAGACAAGTCAATAAATTCCAGAAGCCTAGAATTTTCCCAAATGAG10.9615384615384616No Hit
ACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGCGGCCTTAGCG10.9615384615384616No Hit
CTTTAATACCTCCCACTTCCCTCAATGATCCCCCATCCAGACAGAAGATA10.9615384615384616No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA10.9615384615384616No Hit
GACCTGAATTGATTCACTATAGAAGTCAATTAATTCTATACCAGAGCCAA10.9615384615384616No Hit
CTCTTACAATGAAATGGATGAACTGACCAATCTTACGAAAGGGGAGAAAG10.9615384615384616No Hit
TTTCATTTTTGTTCAAAAAAAAAAAGTCCCTTGGAGTTTTGAAGACCAGA10.9615384615384616No Hit
AGTTATAACATGTTGTTAAAGAAATAACTGACAACCAGCTCCTTTTTCTA10.9615384615384616No Hit
GGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGTAAGGTT10.9615384615384616No Hit
CAGCAGAGTGGCTAACGGGCTCATGGGGCAGGTCTGTTCCAGTAGCTGCT10.9615384615384616No Hit
CATTAATTTCTCTTTTTTAAGATTTATTTATTTGAGAGAGAGAAAGAGAG10.9615384615384616No Hit
GTGAATGCTATGCTTTCACAGAGGTTTTCAGCTTGGCGCGAGCCGAACAA10.9615384615384616No Hit
CTACAAATCATCCACAGACAAAAATGAATACCAATCCTTCTCCAACTCTG10.9615384615384616No Hit
GCCTGTTTGTGATCTCTCTCTGTCAAATGAATAGATTCTATTTATTTATT10.9615384615384616No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA10.9615384615384616No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA10.9615384615384616No Hit
GAGCCACTACCATTTGGACGGTGCAGCCTACCTGCAGTGACCATGATCTG10.9615384615384616No Hit
CGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTAC10.9615384615384616No Hit
GGTACATGAAAGTGCTGAAGCAAAATGCCATTAATAGTCCTGGTATTATT10.9615384615384616No Hit
ATAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCCAAAATAGGT10.9615384615384616No Hit
CCCCTGTGATGTTCTGAAGCACGTTTCTGAAGAATTGGCAGTCGAGCCGT10.9615384615384616No Hit
CAGGGAGCTCGTGCTGCTCTGACGGCCGAGTCACCCGGGAGCAAGTCAGA10.9615384615384616No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTTCTTCTACTAACTTTGGACTTT10.9615384615384616No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC10.9615384615384616No Hit
CCCTAGATGTGAAACCCAAGCCCCCCTGCCAGTGGTTTCCGCTAGCCAGA10.9615384615384616No Hit
TTCCACATCCATGCGCAATCAGTGGTACTAAGGGCTAGTGCGGTCCTGGC10.9615384615384616No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG10.9615384615384616No Hit
CACTTGTAAACAGCTGTAATCCTAACTCATCATACACACGATAATTAACG10.9615384615384616No Hit
GGGTGGCAGTGATTTGGTAGCGGCGCTATCAAATGCTTTTTCTGCATCTG10.9615384615384616No Hit
GTTTCTTGAGTTAGGCCTGTATCACTATAAACTTCCTTCTTAGAACAACT10.9615384615384616No Hit
CACTTGGCCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTAC10.9615384615384616No Hit
CAGTGGTACTCAGTGATAGTGCGGTCCTGGCAATGTTTAAGCAGAAATAA10.9615384615384616No Hit
GAAATATACATGAGTTTAATAGGCATTAGCGTAATTGGGCAACTAACCAA10.9615384615384616No Hit
CCCCTCTCCTCCAGGGTGTCAGTGCAGCTAGGGGGTCAGCGTCTCCAGGA10.9615384615384616No Hit
CCTAGAACGTTCTCCAACTTAGGTCATATGTAAGGCCACAAGACAAGTCA10.9615384615384616No Hit
TTGGTGGGCTGTATCTCATTTAATGTTCAGAACCAGCCTGCTGTGCCCGC10.9615384615384616No Hit
CCCCAGATTTGAGGAGCGACAATGAGAAGATGAGAGAAAAGTGAAATTGG10.9615384615384616No Hit
CCTGAATCTCATTTTTCTGATGGGACGCCATGCCACTATTCGGGTAATGG10.9615384615384616No Hit
CTCCTGGGTATTTACCCCAAAGATACAGATGTCGTGAAAAGAAGGGCCAT10.9615384615384616No Hit
GGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGA10.9615384615384616No Hit
TCACCAGGCCTCAACCCCTCACCCCTTGTCTGGAGCCTGGCAGCCTGGGC10.9615384615384616No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG10.9615384615384616No Hit
AACCACTGATATGCAAAGGCTCTTAAGAATGTACTATGAACAATTACACC10.9615384615384616No Hit
GACAAATACAATCAAGACTGAATTCTTTAATATATATTCACTATTGTTAT10.9615384615384616No Hit
TGATAGCAGAGATCATTTGCTTCTCCTACTCCTCCCATAGGTGACCTTCC10.9615384615384616No Hit
GTGATATTGACAGTGCAGCAGTGTGTCACGTCAAGTAGACGAAAAGTGAT10.9615384615384616No Hit
CACAAAAACAAAATAAAAGGAAAAAAACTTGCTATCCAAATGCCCTAGTC10.9615384615384616No Hit
CATTTGCACACAGCAGGTTCTTAAAAATGTTGTCGTCAGCAGCCATGATT10.9615384615384616No Hit
TCTTAGTGTAGGGCTTGGCTACTTTAATATTTAGAAACGATATGGAGCAC10.9615384615384616No Hit
GAATATAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCTT10.9615384615384616No Hit
TAATTGATATGCAAAAAACCACGGCGTTTTTCAGCGGGTTATTTCTAATT10.9615384615384616No Hit
ACAAAGAGCTGGTTCTTAGAAAAGAACAAAGCCGACAGAGTCTCAGCCAG10.9615384615384616No Hit
AATATAACTGCATTGCACAATGGCTGAGTTATGTCTAGAAATACAGCCCT10.9615384615384616No Hit
TTGCTCACCATTTAAAAGTCAGCGGGATCTCCTCCCATGCTTCCGGCTTT10.9615384615384616No Hit
TTATGAATGTTTGCCCCTGAACTGCATGCTTTTGTTAGGTGTATTTTACC10.9615384615384616No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG10.9615384615384616No Hit
GACCCTAGGGTGGCCACAGAGTTCCGGGAGTCCTAGGGAGATGGTGATGT10.9615384615384616No Hit
GCCACAGTTTAACCGTTTTGAACTGGACCTTAGAGAAGAGGTCTGTTCTG10.9615384615384616No Hit
GCCCAGGACCAGTAAAGAAGGTCATGTCCCAGCTGAGCCAGCACCGTCAT10.9615384615384616No Hit
ACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGATAGAGTCTCAGCCAG10.9615384615384616No Hit
CTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTA10.9615384615384616No Hit
ATCTAGCACAGAGCAAGCATGTAAGGAGAGGCAGGTCTTACATGGGGTGC10.9615384615384616No Hit
GAACATCCTCACTGGTCCCAGCTGTAAAATGGGCCAATTCCGTGAAACGA10.9615384615384616No Hit
ACTCCATTGGAACTGAAAATAAGAAGATCATCTGTTAGTTCTGCAGCCAT10.9615384615384616No Hit
CTCCAGAACCTTCTGGTCTATCTTGCGAATCAGGTTGTTATCCAGGTTGA10.9615384615384616No Hit
AAGTATTTCTGTGTTCCTATTTTGGTTTGCCCATGAACCCAATTGGTTGT10.9615384615384616No Hit
GAAGCAGGCTCCCTGCTGAGCATGGGTCGTAGGGCTCCATCCCCAGGACC10.9615384615384616No Hit
GTATCCAACTCATTAAAGCAGAATTGCCCAGACTTCTTCTTTTTTTTTTT10.9615384615384616No Hit
GTTGTGGGGGATGCTCACTCCACTGTGATCCCACATTCTTGTCCTGAGCA10.9615384615384616No Hit
ACTAAAATTGAATTCTAAAGAAACAGACATCTGACTGTTCAACTGTCTAC10.9615384615384616No Hit
CAGGCCGGGGTGCGTGTGTCCCGCCCCAGTGAACAGGGTCTAGGCCTGAG10.9615384615384616No Hit
CCCTTAGAAGTCTGCTTCTTGTATTCAGACTTCCATTTCATTGACGAACG10.9615384615384616No Hit
GTCTAATAACAAATCTCAATGTTCCTGTCAACTTCAGTTCTAAAGACTAA10.9615384615384616No Hit
GTACTGCCAATTGTGACTTACTAATTAATTTGGCCTTCATTGAACTTCAC10.9615384615384616No Hit
ATTGTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTGGCTCT10.9615384615384616No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10.9615384615384616No Hit
GTCTTGTATTATGATGTTCTGCCACTGGAGGGCAGAAGACCCGCTCCTAC10.9615384615384616No Hit
AAAGACCACATGAAGACACAGGGAGAGGGCACGACTGTCTGCAAGCCAAG10.9615384615384616No Hit
CAAAACTCACAGAATGCAGTAAAACAGGTTCTAAAATGGGGCACCCGATT10.9615384615384616No Hit
CCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTG10.9615384615384616No Hit
CTACATTATTGTGTGGTTTTTAGTGAAAATTGGCTAAAGATCACACTTTC10.9615384615384616No Hit
ATCCCCGACAGTCCCAATGCTGTGGTCCAAGGGGTACCCGAGTCTCCAAC10.9615384615384616No Hit
CAACAGAAACATCAACCTCAGACAACCTGTTACTAACATGGACTTCACAG10.9615384615384616No Hit
GGCATATGTTGCCAAACTCTAAACCAAATACTCATTCTGATGTTTTAAAT10.9615384615384616No Hit
GTGGTAGGGCTTGCATTTGAACTCAGTTGTTTCTATATCAAAGGGTCTGG10.9615384615384616No Hit
ATTCAAAGTATTAGTGCAAGGAATAGAAAAATATGTGAGGGAGAAAGTAA10.9615384615384616No Hit
GTAAAATACAATAACACAAAACTCACACCTTAATCATTCTAAAGAGTAAG10.9615384615384616No Hit
TGTGAACCGAATGTCTTAAGCCACTAAGAACAGACAGTAAACATTTCCTC10.9615384615384616No Hit
CTCGTATTGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTA10.9615384615384616No Hit
CCCTTTGGAAGACCTTTGCTAAAGCACTTAAAAGGCTGTTTTTCTCAGGG10.9615384615384616No Hit
CCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCA10.9615384615384616No Hit
GGACAGAGGGAGAAGACGACTCCCCACTGAGCAGGGAGCCTGACCTGGGG10.9615384615384616No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT10.9615384615384616No Hit
ATCTATGATGAACACAAACATAAAAATCCTCACCCAAATATCTGCAAACC10.9615384615384616No Hit
GACTTAGACTCTCCTGAACGAACGTTGAAGACGACGACGATGATAGGCCC10.9615384615384616No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT10.9615384615384616No Hit
AGTCCATTAGTCGGTGGAGCTCTGTGACTCTTGATCTTGTGGTTTTGAGG10.9615384615384616No Hit
ATATACATTCATTACAACCATTTTTCCTTTTCATCTTTGAGCACAGTTCT10.9615384615384616No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGCGCTCTTCAATGG10.9615384615384616No Hit
TGGCAACACAGGCACCAGGGCAGTAGAAGCACTGCAGGGTGGCGCAGCAG10.9615384615384616No Hit
ATATTTAACATTGGCGGACGGAACATGTAAATATGGTTAAGATGGCCAGT10.9615384615384616No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG10.9615384615384616No Hit
TCCCTAGGCAGGGTAGTCTAATGAGTCTTAGAAGATAGACAGTACGGGTT10.9615384615384616No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers