FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746468
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC47320.27094684815295783No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT37060.21219970821108658No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT32630.1868342277098693No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG27810.15923566878980894No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT26790.15339530984821936No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT25700.147154141959658No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG24740.1416573335440443No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT22930.13129355934377268No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT22900.13112178408078476No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG22570.12923225618791756No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC20900.11967009988158958No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA20610.11800960567270628No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA20150.1153757183068914No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC19470.11148214567916503No Hit
ATTGTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTGGCTCT19130.10953535936530186No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC19120.10947810094430588No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT18230.10438210147566403No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA17690.10129014674188133No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA17540.1004312704269417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA14150.053.8071371
ACATGGT14750.052.1114655
ACTACGC701.6949163E-551.793951
ATCTATG8900.044.8104971
GACGACT1652.7284841E-1143.9476364
GCGAAAG3250.042.3925672
TTACGTG1755.2750693E-1141.4363442
GACATGG19000.039.6916584
GTATACG1105.2608157E-639.5517431
GTTACGT2057.2759576E-1238.9086231
AGCGAAA3550.038.8089831
GCGTCGG750.001483366138.6683859
GGTCCTA24400.037.737339
CGAAAGC3900.037.186463
GCTACTA3500.035.215859
CATGGTC22300.034.793526
GGTTATA7100.034.7238271
AGTCCGC2302.7284841E-1134.67347145
ATGGTCC26650.034.5522427
TGGTCCT26700.034.4875378
Produced by FastQC (version 0.11.5)