FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675204
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC35250.2104221336625271No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT28540.17036731048875242No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT21660.12929768553561238No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC19010.1134787166219756No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT18660.11138941884092923No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT18110.10810623661357065No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC17510.10452458327463401No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG17360.10362916993989985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG554.3964974E-879.090126145
ACCGTAC902.0469088E-856.40181
GTTACGT2150.053.965841
TTACGTG2200.052.7393462
CCGTACA1004.6973582E-850.761622
ACTACGC752.5478776E-548.34441
CGAAGCG752.5514773E-548.3328559
CCGACTA4750.036.6399654
CGTATAC1406.601331E-736.2582973
TAGTACG850.00274264434.117319
ATGCCGG2353.45608E-1133.935833145
GTTGACA11550.033.9038661
ACGTGTT3450.033.6308864
CTCCGAC1301.655558E-533.461205145
AGTCCGC1601.878263E-631.718437145
GCGAAAG3900.031.6097972
AGCGAAA3900.031.6097971
CCACCGA5550.031.358531
TACGTGT3750.030.9404163
ACTCGCG1652.387882E-630.757273145
Produced by FastQC (version 0.11.5)