FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356960
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC23160.17067562787407145No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT18280.1347128876311756No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG16380.12071100106119562No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16050.11827909444640963No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT14740.10862516212710766No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT13570.10000294776559368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2900.052.510851
CTAGTAC701.6938668E-551.7983253
GTTGACA11700.047.103751
GCGAAAG3600.044.3163452
ACATGGT12750.043.796595
GTATATC2750.036.916721
GTTACGT1803.1959644E-936.2574921
GATAGCG3850.035.778698145
TTACGTG2052.910383E-1035.3744662
TAGTCGG2103.783498E-1034.5271268
CAAGCGG850.00273905734.1259543
GTACTAG850.00273955234.1246951
CGACCAA850.002742523634.117153145
CCGAGGT4100.033.6069834
CCGACTA3700.033.3201524
GACATGG16750.032.9047474
CGAAAGC4650.032.749913
GGTCCTA19550.031.8944729
AGTCCGC1652.3875273E-630.757132145
TGACATG19200.030.5933863
Produced by FastQC (version 0.11.5)