FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400656
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT24410.1742754823454153No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18640.13308049942312744No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC18130.12943934841959767No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG17730.12658354371094688No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG16920.120800539175929No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG16220.1158028809357901No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT15930.11373242252201825No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC15360.10966290081219085No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15330.10944871545904204No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA14270.10188083298111741No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA14220.10152385739253607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT2100.058.7064444
GTTACGT2350.055.5470281
TTACGTG2450.053.27982
GCGTAGT553.215126E-452.741623
AGACGAC2600.050.2059673
ACGTGTT2650.049.2586824
GTCTACG450.00962958148.3464851
TACGCCT450.00962958148.3464854
ACTACGC1056.8943336E-848.3464851
CGAAGCG450.00963909448.3344049
AAGACGA2650.046.522092
TACGTGT2950.044.249333
AGCGAAA3000.043.5118331
TATACTG5250.041.4398465
CAGTGCA7400.041.1598472
CTACGCA1303.6918937E-739.0490842
GTTATGT8550.039.016461
GCTACTA2109.094947E-1237.9770289
ATGCCGG1001.3918446E-436.24951145
GTTGACA12100.034.7615241
Produced by FastQC (version 0.11.5)