FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331264
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC25110.1886177347242921No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT19680.14782943127734244No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG16770.12597050622566222No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC14840.1114730061054757No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT14010.10523832988798615No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC13470.10118203451757127No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT13420.10080645161290322No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA13370.10043086870823517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2700.045.6612171
CTCCGAC1201.9743311E-742.29106145
CGAAAGC3200.038.5266533
GTTACGT1406.5970016E-736.260381
ATGCCGG800.00203699936.249485145
GCGAAAG3450.035.7348672
TTACGTG1458.6818363E-735.010022
GATATAC1905.4151315E-934.3519363
CCTATAT1801.2896999E-732.2314451
GCATACG1601.8735427E-631.7278331
GCTACTA1851.6439844E-731.3520819
GGCATGC3250.031.230326145
CCGACTA2807.2759576E-1231.0803244
TATTGGG8750.030.665922
CAGTGCA6350.029.6935392
GGATAGT2252.8107024E-829.0083031
GTATTAG2802.6193447E-1028.4902971
TATTAGC2054.030735E-728.3007832
CCACGAC2104.9891605E-727.618652145
TATACTG4500.027.396735
Produced by FastQC (version 0.11.5)