FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603266
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT9860.16344365503774455No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG6940.11504046307930497No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT6920.11470893436726089No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG6760.11205670467090802No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC6620.10973600368659928No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC6620.10973600368659928No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC6440.10675224527820232No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC6280.10410001558184946No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGG350.00356484962.147128
GCGAAAG1301.8189894E-1255.786932
ACCGTAC400.0060441254.3877451
AGCGAAA1353.6379788E-1253.716291
CGAAAGC1405.456968E-1251.8021473
ACGTAAC604.9357803E-448.348673
GACGACT1056.883238E-848.3486674
CACGAGC1109.951691E-846.135704145
CCGTACA657.322804E-444.6295432
CAGCTAC1201.9706386E-742.2945677
GCTACTA1201.97193E-742.291069
TTAGGTA1755.2750693E-1141.441724
CAGTGCA3350.041.1324542
AAGACGA1252.709785E-740.6128852
AGACGAC1451.9203071E-840.012693
TATACTG2950.039.334515
GTACGTG951.02698716E-438.166841
GTTGACA4950.036.6247441
GTGCAGC4000.036.2615054
AATCGCT1001.3882012E-436.2615052
Produced by FastQC (version 0.11.5)