FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2254_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2254_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402576
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC27750.19785024127034825No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT18090.1289769680929946No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT14870.10601921036721006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC300.001935120172.5074161
GCGACTA604.9432734E-448.3382764
GCGTAGT450.009636044548.3382763
TACGTAC450.009636044548.3382764
AGCGAAA3350.047.616811
CTCCGAC951.921504E-645.78926145
GCGAAAG4000.039.879082
GACGACT1157.13975E-637.8299564
AGACGGC7600.037.203773145
TTACGTG1209.559892E-636.2537082
CGAAAGC4450.035.8463633
GTATTAG3750.034.803561
ATGCCGG4400.034.60211145
AAGTCGA1051.8529821E-434.527341
TCTCGTT3600.034.23595145
TATACTG6900.031.5249635
GTAGAAA8100.031.3303622
AAGGTCG1703.012965E-629.8528029
TACGTTC2202.2633685E-829.6621254
TTTACGT2451.9717845E-929.5948642
Produced by FastQC (version 0.11.5)