FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029873
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC31050.15296523477084528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC5200.039.038017145
GTGATCG2808.20728E-925.8925619
GCGAAAG6300.025.3240362
GTCCTAT20900.024.9825611
GAATAAC14950.024.2537881
AGCGAAA7150.023.3277341
TCTCCGC8850.022.937592145
TCCTATG21700.022.724792
GTATATA9400.022.372831
GTATTAT17700.022.1243881
GTATTAG7300.021.8549881
CGTATAC1700.003089223521.3290653
GTTGACA12600.021.2952121
GTACCGC2404.357001E-521.1513236
AGACCGC3457.514973E-821.014252145
CGAAAGC8100.020.5917643
TATACTG11300.019.8945435
GATAGCG2959.571122E-619.660793145
CTATACT5950.019.500864
CGCTAGT1900.00589031919.078732145
Produced by FastQC (version 0.11.5)