FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685157
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC32220.19119880224809913No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT18670.11079086399664838No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA350.003563736662.1568955
CACGAGC6150.035.365448145
CTATGCG1801.2910641E-732.2285464
GCGAAAG7100.031.6611422
CGAAAGC7550.029.7740543
TCTCCGC10600.028.726086145
AGCGAAA8200.027.4139161
TCTATAC18450.025.940053
TACCGGA1701.03032835E-425.5932582
TATACTG7250.025.005655
GAATAAC9550.024.2979621
GTGATCG2701.649496E-724.1678249
GTTCTAT20750.023.76371
CGTAGAC1550.001809121523.38683145
GTATTAC5000.023.2045541
GTCTAAT5950.023.1558041
ACCGTAC2202.2296645E-523.06868
TATACCA21600.022.1577825
CTATACC21000.022.0995734
TATAATA4358.367351E-1121.670922
Produced by FastQC (version 0.11.5)