FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436404
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC33280.13659475193769177No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT30000.12313228840537119No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC27870.11438989592858984No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG26250.10774075235469979No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG1353.6379788E-1253.702953145
GCGAAAG5550.040.5057332
AGCGAAA6050.037.1581541
GACTCGC2052.928573E-1035.365356145
GGTCGTT1854.1873136E-935.2712257
CCGACTA5000.034.80884
CGAAAGC7200.034.244773
GTTGACA19750.033.0463261
TATACTG11800.032.5717935
AAGGTCG2051.1372322E-831.8373155
AAGTCGA2451.9699655E-929.5993211
AGTCGAA2502.4447218E-929.0073322
ACATGGT22000.028.6777045
GCGTTAA2802.6375346E-1028.4834989
GTTACGT2553.0195224E-928.4385621
GTATTAT32150.027.7441841
GCGATAT1050.00770108327.626031
GTCGAAG2756.7575456E-926.3703023
TCTCCGC9100.026.29084145
GCTACTA5300.025.9918029
Produced by FastQC (version 0.11.5)