FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908897
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC33650.17627980975400978No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT25030.13112284214391873No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC22510.11792150126486657No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG22340.11703093461826383No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA21510.11268287393190937No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC20240.10602981721905373No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT19690.10314857218592727No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG19440.10183891535268798No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA19290.10105312125274438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3800.047.705631
GTTGACA19050.044.9159161
GTTACGT1801.8189894E-1244.3132321
GTGATCG1703.8198777E-1142.6477439
ACATGGT20250.042.2542345
GCGAAAG4450.040.7373922
TTACGTG2005.456968E-1239.881912
CGAAAGC4850.038.872713
CACGAGC3750.038.666275145
GACATGG23500.036.719134
GGTCCTA31750.032.4257169
ATCTATG12350.031.1187521
GCAGTGC12150.031.0341821
TGACATG29750.030.4674633
GCAACCG1204.0552957E-430.2135681
TGGTCCT35800.028.9592068
ATGGTCC35850.028.9188167
CACGAAC5600.028.4870784
TCGCGTT2156.1300307E-726.976473145
CCACGAA5400.026.8565033
Produced by FastQC (version 0.11.5)