FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429619
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21400.1496902321527624No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG20610.1441642843303006No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA20200.14129638735914954No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20020.1400373106401076No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT19120.13374192704489796No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA17310.12108121114786526No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA16840.11779362193703358No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16610.11618480168492445No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC16390.11464593013942877No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT15800.11051895644923578No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA15150.10597229051936216No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT15130.10583239310613526No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA14840.10380388061434549No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC14590.1020551629490095No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT450.0096404848.332695145
GTTACGT2100.044.8929181
CAGTGCA9650.038.326282
GTTATGT9500.035.8779831
GATATAC3150.034.5330163
TATAACG1905.4151315E-934.351265
TAACGTC1905.4296834E-934.3428547
GCAGTGC10800.034.245241
ATGCCGG1707.827657E-834.11719145
TATACTG6950.033.3901985
GATAGCG6150.033.007694145
GACGACT2008.933057E-932.6336984
CTACTGA22250.032.5929576
TTACGTG2900.032.5086632
GTAACGG4250.032.4204061
AAGACGA2051.1363227E-831.8377552
ATAACGT2051.1363227E-831.8377556
CTCCGAC1153.163021E-431.52132145
GCGATTA1153.163021E-431.52132145
CATTCTA17050.031.4746652
Produced by FastQC (version 0.11.5)