FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389510
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT5230.1342712638956638No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC4960.12733947780544785No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA4600.11809709635182666No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT4470.11475956971579677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG851.6916601E-1068.243692
TGTACGT350.003564944362.1425258
CGAAAGC954.5474735E-1061.060143
GTTGACA3650.057.609831
ACATGGT4200.050.065695
AGCGAAA1203.607056E-948.3392831
CTATTGG604.9383397E-448.3392831
TAGTCGG450.00963495748.3330768
GTAATAC951.9162198E-645.7951053
GCTACTA951.9179151E-645.789239
GATAGCG1002.7299247E-643.499763145
GACATGG4850.043.355854
CAGTGCA2550.039.8088232
TGGTCCT5850.039.657918
GCAGTGC2750.039.550321
GGTCCTA5700.039.4296159
TGACATG5650.038.5003133
TATACTG1701.8208084E-938.3870775
GGTTATA1354.9562186E-737.597221
ATGGTCC6300.036.8252037
Produced by FastQC (version 0.11.5)