FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144437
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT48630.22677280796777896No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT36380.1696482573281472No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT32610.15206788541701155No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG31420.1465186433548759No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT30490.14218184073488752No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA28260.13178284090416273No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG27890.13005744631341465No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA27790.12959112345105034No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG27470.12809889029148444No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA26590.12399524910267823No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG25640.119565181910217No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT25050.11681387702226738No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC24110.11243044211604258No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG23470.10944597579691079No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG23240.10837343321347281No Hit
ATTGTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTGGCTCT22870.10664803862272475No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT22480.10482937945950382No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA22100.10305735258251932No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA21590.10067910598446118No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT21490.10021278312209686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1250.075.399345145
GTAACGC350.003564218362.155363
ATACCGT502.0087668E-458.011676
CTCCGAC1007.203198E-1057.9995145
CGAAGCG651.0927659E-555.7804575
AGCGAAA3650.055.6276321
TATACCG951.9192594E-645.7986875
GTAGAAA18300.043.191752
GTTACGT2200.042.849531
GTTGACA17550.042.558421
ACAAGGC13700.042.33548
GTCGAAG1755.2750693E-1141.4369053
ATGCCGG3550.040.844715145
GGTGCGA1601.0113581E-940.7808958
CAGTGCA13450.039.8965032
TTACGTG2550.039.811932
CCGACTA6450.039.3494
ACATGGT18800.039.343025
CTTATAT8350.039.0797161
CGAAAGC5200.039.0463183
Produced by FastQC (version 0.11.5)