FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2253_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2253_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611116
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC31400.19489596031570663No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC28320.1757787769471596No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG24240.15045471586155187No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG21610.1341306274656822No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG20980.13022029450393394No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA20410.1266823742052093No Hit
TTAGTAGACAGCTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATCTT19590.12159273447721952No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT18510.11488930654279393No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA18110.11240655545596966No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG17410.10806174105402716No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT17120.10626174651607953No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA16910.10495830219549677No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16800.1042755456466201No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT16440.10204106966847824No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC16380.1016686570054546No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT16200.10055141901638368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA3900.052.055771
ATAAACG450.009635732548.3394
GTAGAAA10200.046.917272
CTCCGAC1402.728484E-1046.606823145
TCTCCGC5450.046.55931145
CCACGAC2700.042.96267145
ATGCCGG3100.042.09649145
GTATTAT12250.041.4321441
GTTACGT1105.2631876E-639.5488661
CACGAGC5150.038.00945145
GTTGACA13650.036.6515121
AGCGAAA3000.036.2531281
ACATGGT13550.035.8540275
GGTCGTT2500.034.801927
TACATCG2302.7284841E-1134.673676145
ATCTATG7150.034.4784971
AACCGAT1905.4242264E-934.3461342
TATTGGG6950.032.3419232
GCGATTA900.003629006732.222145
GTAACAG4750.032.0553971
Produced by FastQC (version 0.11.5)