FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006561
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC31360.15628729951394452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT2850.035.613655145
TCTCCGC8300.031.445448145
CACGAGC6300.029.920317145
GAATAAC16050.029.8187121
GCGAAAG5050.028.7182922
GTCCTAT23300.027.6983591
TGTATCG1100.00966496826.368615
GTATATA12800.024.3600641
GTTACGT1500.001493154624.1712271
CCACGAC4700.023.138052145
TATCGAG6400.022.6605241
GTGATCG3854.292815E-1022.5977259
TATAGGT6100.022.586234
ATAACAG18350.022.1295193
GTATTAT21150.021.9426751
TCCTATG27650.021.7672162
TATAATA13050.021.6707552
TATACTG10100.021.538725
GTATACG1700.00309048221.3275551
GTATATC7050.020.5712571
Produced by FastQC (version 0.11.5)