FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849356
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC42710.230945258781976No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT25470.1377236183839131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC6550.046.488136145
GTACCGC1051.852065E-434.530976
TCTCCGC7200.033.22887145
CCACGAC4050.030.431826145
CTCCGAC1952.6095404E-729.743322145
GAATAAC14250.027.4793821
ATCTATG10800.026.857421
GTCCTAT26350.025.868741
TATAATA8100.025.0669252
TCCTATG27300.024.4373022
GCGAAAG5400.024.1716772
ACCGTTC1500.001494829824.1664528
GTACTAG4251.8189894E-1223.8873041
AGACGCG4650.023.386887145
GTATAAG6700.022.7285941
AGCGAAA5850.022.3123191
CCGTGCA2603.2068547E-622.3074919
CACCGTT2303.1441017E-522.065027
GTTCTAT38800.021.4928591
TCTATGA14850.021.4859352
Produced by FastQC (version 0.11.5)