FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370621
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC29130.12287919494512198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6700.038.9654432
AGCGAAA6750.037.6000751
CGAAAGC7350.034.5328643
TCGCGTT1102.4373227E-432.95415145
GTTGACA13750.031.1152821
CTAGTAC3201.8189894E-1229.4608523
TATAATA17700.027.8604152
GTACTAG3153.45608E-1127.6245441
CGGCGTA2654.5529305E-927.3656673
GTATTAG6200.026.9005151
GACTCGC3255.0931703E-1126.76891145
CCGACTA3357.4578566E-1125.9769614
TACAGCC14750.025.5611747
GTATTAT25050.024.0267371
GCACCGT12500.023.7862386
ACATGGT17750.023.6963565
TAGTACT8900.023.6297914
ATACAGC16900.023.6008646
CTCGGCG3152.8632712E-823.0204541
TCGGCGT3203.3811375E-822.6621932
Produced by FastQC (version 0.11.5)