FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549294
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC19280.12444377890832856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5650.037.2169882
AGCGAAA5400.036.254481
CACGAGC4950.033.686684145
CGAAAGC7000.031.0752663
GTTACGC950.00472523230.5300873
GTATTAG3900.029.7472631
CTCCGAC2202.266097E-829.658926145
GTTGACA10100.029.434331
GAATAAC8400.028.4856621
TCTCCGC6350.027.401423145
TTCGACA1358.078895E-426.855174
TAGTACT4950.026.3668944
TATAATA10550.026.116972
GTGATCG1409.997402E-425.8927149
TATACTG6850.025.4045985
TCGCGTT2451.9133058E-623.673338145
GTTACGT1550.00180763623.3899861
ATACAGC11300.023.1001996
ACATGGT13400.022.7266885
GTGTACG2603.2062035E-622.307579
Produced by FastQC (version 0.11.5)