FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491640
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15580.10444879461532273No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG2700.051.0332532
GTTACGT2900.045.009981
AGCGAAA4300.043.8469281
ATGCCGG1851.8189894E-1243.107548145
CCGACTA4350.040.0115554
GCGAAAG5200.037.65512
CGAAAGC5300.035.576313
TATACTG6150.034.1968655
GATAGCG4050.034.0119145
ACGTGTT3850.033.905894
TATGGAC6050.032.3647165
TACGTGT4450.029.3343123
CTTATAT6750.029.006431
CTCCGAC1504.4005275E-528.999624145
CCACCGA5550.028.7451111
GTTGACA11100.028.0918121
CGTCTTG2654.5620254E-927.358137145
TACATCG1358.0851966E-426.851505145
CAAGCGG1100.00965947426.371253
GACTTAA6500.025.6595341
Produced by FastQC (version 0.11.5)