FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334525
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG16690.12506322474288603No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT13910.10423184279050599No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13810.10348251250444915No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA13560.10160918678930705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1700.068.252081
TTACGTG2100.055.2516862
GTCGTCG450.00964045548.33255145
ACGTGTT2450.047.3585854
GTTGACA11500.044.771881
CCGAGGT4400.042.851454
GCTACTA2700.040.280149
TACGTCT750.00148177238.6761784
AAGTCGA1701.8262654E-938.3917961
ACATGGT13350.036.9379275
CTCCGAC1406.612663E-736.24941145
CAGTGCA8650.036.049332
CGAGGTG5350.033.886845
GCAGTGC9850.032.3937531
GTATTAT13400.031.3883191
CAGCTAC3700.031.3520167
CACGAGC3950.031.202023145
AAGACGA1652.3826415E-630.7651422
AGTGCAG10200.030.5712453
TATTGGG8550.030.5338252
Produced by FastQC (version 0.11.5)