FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943286
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC37270.1917885478514228No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT36450.18756889104331528No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC36240.18648824722660484No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG33360.1716679891688614No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG30230.15556125037693885No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA29160.15005511283465223No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT24740.12731013345436545No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC24010.12355360971056242No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT23170.11923103444372057No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT23030.11851060523258028No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA22790.11727558372776833No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA21710.11171798695611454No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG21450.11038004699256826No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT21200.10909356625838913No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA20410.10502828713838312No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA20300.10446223561534432No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT20150.10369034717483686No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA20090.10338159179863386No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT19600.10086008955964279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC2000.050.74931145
GACGACT1301.3096724E-1050.2032434
GGTTATA5550.045.7283331
TTACGTG1751.8189894E-1245.5813642
GTTACGT1751.8189894E-1245.5790141
GTTGACA15950.045.0074921
ACATGGT16800.043.165285
GTATTAT23400.041.833891
GGTCCTA24950.036.90529
ATCTATG11900.036.5607071
GACATGG20550.034.934624
TATGGAC7350.034.5322235
GATATGT9150.034.0767141
AAGACGA1956.9849193E-933.4688342
TGGTCCT27650.033.3031548
TATTATT24900.033.2000052
GCGAAAG3700.031.3565711
TCTATGA15300.031.2813262
GCAGTGC15150.031.110791
ATGGTCC29950.030.745657
Produced by FastQC (version 0.11.5)