FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852860
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC19290.22618014680017823No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17200.2016743662500293No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT10040.11772154867152873No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC9110.10681706258940506No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA9100.10669981005088759No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT8960.1050582745116432No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8900.10435475928053842No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC8840.10365124404943366No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC8800.10318223389536384No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC8770.10283047627981147No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT8720.10224421358722416No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG8570.10048542550946228No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG300.001933918772.5163044
TATCGGC300.001935718972.49939
TGCGTTA955.456968E-1268.683558
CTCCGAC554.0613268E-665.90845145
CGATTGA350.003566460762.1422549
ACGATTG400.006050813554.3744748
GCGTTAA1206.002665E-1154.374479
TACTGCG1258.913048E-1152.2117355
GTTGACA7300.051.6554451
ACATGGT7400.050.9573975
ACTGCGT1501.0913936E-1148.3442046
AACCGAT1109.939504E-846.1467362
GGTTATA2700.045.6584131
CACGAGC2950.041.779255145
GGTCCTA12800.037.9488539
TGGTCCT13200.037.348128
CCGATGA1406.596474E-736.2581524
GATAGTG2002.237357E-1036.258152
GATAGCG1604.5887646E-836.24965145
TATACTG3250.035.7003335
Produced by FastQC (version 0.11.5)