FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882299
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA43540.49348350162473265No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA41320.4683219634160301No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT38380.4349999263288296No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG36170.40995172838232846No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA35530.4026979516014413No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA34820.3946507929851445No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG34000.38535689148463276No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT30360.3441010360433368No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT28330.3210929628164602No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG28100.3184861367858288No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG21510.24379490399513093No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC19120.21670658132900528No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT18900.21421309556057527No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT17410.19732539649257225No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG16270.18440460660161692No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT16210.18372456502840873No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC16180.18338454424180464No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG14830.1680836088446207No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT14120.16003645022832394No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC13730.1556161800024708No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC13680.15504947869146402No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT13460.15255599292303404No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG12920.14643561876416045No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC12570.14246870958711277No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA11950.1354416133306283No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG11890.1347615717574201No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC11700.1326081067755942No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC11570.1311346833669765No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG11390.12909455864735198No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG11340.12852785733634517No Hit
GGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGA10880.12331420527508248No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA10830.12274750396407566No Hit
TAAAAGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAGA10780.12218080265306887No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG10480.11878059478702797No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA10390.11776053242721572No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT9860.1117534985305435No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC9860.1117534985305435No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA9780.11084677643293261No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA9560.10835329066450264No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG9380.1063131659448781No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC9050.10257293729223312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2550.082.477421
GCAGGTA5200.068.339212
GGTCAAA8500.068.257171
GGTACTG5350.067.782585
GACGACT554.053487E-665.930224
GTACTGA5700.063.6204876
ACAAGGC10600.062.2428788
AGACGAC606.79201E-660.4360353
ACTGATC6300.059.843498
GGGTTAC502.0069316E-458.0185931
ATGCGCC502.0103084E-457.998867145
AAGGTCG6950.057.3794829
CGAAAGC3750.056.084643
GTAGAAA15450.055.8593252
GCGAAAG3800.055.3466872
GCTCGTT400.006051119454.373936145
CAAGGCA12350.053.4260649
GACAGTA5800.051.2519047
CAATCCA9500.048.0970885
TTGGACA6500.047.9769134
Produced by FastQC (version 0.11.5)