FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988040
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT26800.13480614072151467No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC22790.11463552041206414No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG651.0941167E-555.76857145
GACCGGC4850.050.824146145
GCGTTAA3000.048.332769
TGCGTTA3200.045.3119668
TACTGCG3300.043.949935
AGCGAAA5200.040.4423871
CACGAGC5750.040.34735145
GACGACT2057.2759576E-1238.9117664
CGAAAGC5850.035.948793
GCGAAAG5850.035.948792
GTTACGT2206.220944E-1032.962451
TCTCGTT4450.032.583885145
TATACTG15100.031.6963395
ATGCCGG4350.031.666294145
TTACGTG2351.2605597E-930.8584612
GCGGACC2151.812623E-830.348489
GTATTAT24150.030.3281821
GTTGACA14150.030.236931
AAGGTCG1952.6097769E-729.7432389
TCTCCGC6600.029.658741145
Produced by FastQC (version 0.11.5)