FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2251_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2251_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427423
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT19740.13829117227339058No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18420.12904373826118815No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT16830.11790478365558071No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT14830.10389352000072859No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA14610.10235228099869484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG451.1941024E-464.44368145
GCGACTA350.00356351962.157364
AGTCGAA1101.6734703E-952.739582
TCGCGTT604.946086E-448.33276145
GTCGAAG1402.728484E-1046.6180233
AAGTCGA1255.187758E-946.410831
CACGAGC4550.044.614857145
GTTGACA10550.043.991311
ACATGGT11600.038.7590455
TCTCCGC5800.037.499554145
AAGGTCG2302.7284841E-1134.6820075
GTAGAAA10100.034.4634862
GGTCGTT1901.3242243E-934.3416987
TACTGCG1751.0076474E-733.1505935
GATATAT6500.032.3537031
GTATTAT15850.032.0263981
GACATGG14450.031.1145274
CCGACTA3500.031.0786824
AGCGAAA2859.094947E-1230.5334421
GCGTTAA1703.0131814E-629.8525899
Produced by FastQC (version 0.11.5)