FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2248_d08.2_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2248_d08.2_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360107
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC68420.2899021103704197No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG60020.25431050371868735No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT55890.23681129711491897No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA53650.2273202020077903No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG44590.18893211197627904No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA38050.16122150394028745No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT25670.10876625508928198No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT25660.10872388412898229No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA24980.10584265882860396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG15250.063.7203645
GTACTGA16950.057.3307466
GTAGAAA21150.055.200172
GCAGGTA17600.054.385882
ACTGATC20900.049.9547358
ACAAGGC20050.046.64838
AGCGAAA6200.045.6139641
AGGTACT22450.045.221584
GTATGGA23050.044.0435181
AGCAGGT22800.042.300131
GACAGTA14300.041.5755777
ACCGTAC700.00105589441.436861
TGGATAG24300.040.883534
CAGGTAC24300.040.585113
TTGGACA16450.039.674434
ATAGCAA25400.039.39187
ATGGATA25650.039.2971953
GCGAAAG7350.038.4770852
GGATAGC26400.037.9069185
TAGAAAC32100.037.27463